Document Detail


A new embolus injection method to evaluate intracerebral hemorrhage in New Zealand white rabbits.
MedLine Citation:
PMID:  20599833     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rabbit large clot embolic stroke model has been used for over 23 years to study methods to manipulate hemorrhage and to test drugs and devices for safety, because the rabbit model is particularly sensitive to embolism-induced hemorrhage. This study refined the original embolization procedure using an automated, pump-assisted injection method to introduce large blood clots or macroscopic emboli into the middle cerebral artery (MCA) via an indwelling carotid artery catheter. The study shows that rapid injection of blood clots (3 ml/30s) produced a model where there is a high hemorrhage incidence rate (79%) and a high stroke success rate (63%), compared to a low stroke success rate (19%) with no hemorrhages when clots were injected at a slow rate (3 ml/90 s). The rapid injection method, which produces a high hemorrhage rate, is particularly useful to study neuroprotective agents to attenuate embolism-induced hemorrhage. In addition, we show that manual injection of blood clots, which produces a lower baseline hemorrhage rate (41%) with a similar stroke success rate (65%), may allow investigators to study pharmacological agents to either up or down-regulate hemorrhage incidence. Lastly, we show that in the rabbit embolic stroke model, hemorrhages are adjacent to areas of 2,3,5-triphenyltetrazolium (TTC)-negative tissue, normally associated with infarcted or ischemic tissue. Thus, there is clear separation of ischemia and hemorrhage in the model, suggesting that therapeutics that are neuroprotective may also be useful to limit the evolution of ischemic damage associated with a hemorrhage, if not attenuate hemorrhage itself.
Authors:
Paul A Lapchak
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-21
Journal Detail:
Title:  Brain research     Volume:  1349     ISSN:  1872-6240     ISO Abbreviation:  Brain Res.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-03     Completed Date:  2010-11-22     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  129-36     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Behavior, Animal
Brain / pathology
Cerebral Hemorrhage / classification,  etiology*,  pathology*
Computer-Aided Design
Disease Models, Animal*
Embolism / complications*
Male
Rabbits
Radioactivity
Tetrazolium Salts / diagnostic use
Time Factors
Grant Support
ID/Acronym/Agency:
U01 NS060685/NS/NINDS NIH HHS; U01 NS060685/NS/NINDS NIH HHS; U01 NS060685-01A2/NS/NINDS NIH HHS; U01 NS060685-01A25444/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Tetrazolium Salts; 7OL20RET2I/triphenyltetrazolium
Comments/Corrections

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