| A new digitized method of the compulsive gnawing test revealed dopaminergic activity of salvinorin A in vivo. | |
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MedLine Citation:
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PMID: 20217638 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The compulsive gnawing (CG) test has been used for numerous years as an assay to determine the dopaminergic activity of various compounds. We developed a new method of quantification via a digitization step which allowed a more precise measurement of the gnawing activity. It was the aim of the present study to explore possible dopaminergic effects of salvinorin A (SA), the major active compound of Salvia divinorum, using the new digitized CG test. A group of experiments using male C57BL/6 mice were performed to validate the new method of quantification showing only significant increases of gnawing when the dopamine reuptake inhibitors buproprion (20 mg/kg, p. o.) and nomifensine (10 mg/kg, i. p.) were given concomitantly with apomorphine (10 mg/kg, i. p.). Different concentrations of the SA (1.0, 2.5, 5, and 10 mg/kg, i. p.) were tested with positive dopaminergic activity when administered with apomorphine which differed from the semisynthetic counterpart U-69593. Furthermore, the activity observed with SA was unsuccessfully antagonized by the κ-opioid receptor antagonist norbinaltorphimine (NorBNI; 10 and 20 mg/kg, i. p.), while the dopamine antagonist haloperidol did successfully block (0.06 mg/kg, i. p.) the gnawing activity seen with SA. Our data further strengthen the argument that salvinorin A is not a selective κ-opioid receptor agonist and is the first in vivo study that veers from salvinorin A acting solely like its synthetic counterparts. Furthermore, the digitized CG test system used in this study provides a new computational method to accurately detect behavior associated with dopaminergic neurotransmission. |
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Authors:
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Stephen M Phipps; Veronika Butterweck |
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Publication Detail:
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Type: Journal Article Date: 2010-03-09 |
Journal Detail:
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Title: Planta medica Volume: 76 ISSN: 1439-0221 ISO Abbreviation: Planta Med. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0066751 Medline TA: Planta Med Country: Germany |
Other Details:
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Languages: eng Pagination: 1405-10 Citation Subset: IM |
Copyright Information:
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© Georg Thieme Verlag KG Stuttgart-New York. |
Affiliation:
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Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville, Florida 32610-0494, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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