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New Computerized Color Image Analysis for the Quantification of Interstitial Fibrosis in Renal Transplantation.
MedLine Citation:
PMID:  21926945     Owner:  NLM     Status:  Publisher    
BACKGROUND.: Chronic allograft injury, the primary cause of late allograft failure in renal transplantation, can be diagnosed early at a preclinical stage by histopathological changes such as interstitial fibrosis (IF). Currently, assessed by semiquantitative analysis in the Banff classification, IF quantification is limited by pathologist's subjective interpretation. METHODS.: We have designed algorithms dedicated to quantify IF by computerized color image analysis. This innovative and objective software automatically extracts the green areas characteristic of IF in Masson's trichrome based on color image segmentation followed by removal of nonspecific IF staining (capsula, sclerosis glomeruli and normal glomeruli, normal basement membrane) and computes an index. It also counts automatically the number of glomeruli. Sixty-seven Masson stained renal transplant biopsies at various IF stages were imaged using a digital color camera mounted on a microscope. We tested the robustness of the method against varying acquisition parameters. RESULTS.: We demonstrated that the parameters do not have an impact on this quantification and that the algorithm is able to handle biopsy color variations. The intra- and interobserver reproducibility was good (P<0.003). The kappa coefficient that was performed on another set of 90 biopsies to evaluate the concordance of our method with an expert Banff quantification was 0.68, indicating a substantial agreement. Finally, the computerized IF correlated with renal function. CONCLUSION.: This study demonstrates that computerized color image analysis is a reliable and reproducible method to evaluate renal IF in routine practice and in multi-centric studies.
Vannary Meas-Yedid; Aude Servais; Laure-Hélène Noël; Clarisse Panterne; Paul Landais; Nicolas Hervé; Nicole Brousse; Henri Kreis; Christophe Legendre; Eric Thervet; Jean-Christophe Olivo-Marin; Emmanuel Morelon
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-16
Journal Detail:
Title:  Transplantation     Volume:  -     ISSN:  1534-6080     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
1 Institut Pasteur, Quantitative Image Analysis Unit, Cell Biology and Infection Department, CNRS URA 2582, Paris, France. 2 Paris Descartes University, Faculty of Medicine, Department of Nephrology, Necker Hospital, Paris, France. 3 Paris Descartes University, Faculty of Medicine, Service d'Anatomie Pathologique, Necker Hospital, Paris, France. 4 Centaure Foundation Nantes, France. 5 Paris Descartes University, Faculty of Medicine, AP-HP Service de Biostatistique, Necker Hospital, Paris, France. 6 Paris Descartes University, Faculty of Medicine, Service de Transplantation Rénale Adulte, Necker Hospital, Paris, France. 7 INSERM U557, Paris, France. 8 Université de Lyon, Service de Néphrologie, Transplantation et Immunologie Clinique, Hôpital Edouard Herriot, Hospices Civils de Lyon, Lyon, INSERM U 851, France.
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