Document Detail


A new approach to the investigation of sexual offenses-cytoskeleton analysis reveals the origin of cells found on forensic swabs.
MedLine Citation:
PMID:  20102457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There are forensic inquiries in which an identification of epithelial cell types would provide important probative evidence. In cancer diagnosis, this information is yielded by histological examination of cytokeratin (Ck). Therefore, we tested 19 antibodies against different Cks (Ck1, Ck2e, Ck4, Ck5-6, Ck7, Ck8, Ck9, CK10, Ck13, Ck14, Ck15, Ck16, Ck17, Ck18, Ck19, Ck20, Ck903, PanCkAE1_3, and CAM5-2) on histological sections of epidermis, buccal mucosa, vaginal mucosa, penis, urogenital tract, and rectum and could identify two antigens unique to buccal-cell and vaginal-cell (Ck4) and skin epithelial-cell (Ck10) cytokeratin. Subsequently, we developed an immunocytological technique for distinguishing swabbed skin and mucosal cells up to at least 1 year after sampling. By the detection of the Ck4 and Ck10 mRNAs in biopsy and laser capture microdissection collected samples via quantitative real-time polymerase chain reaction, we were able to confirm our immunological findings. Hence, this study offers techniques to discriminate between skin and mucosal cells (buccal and vaginal) in forensic casework.
Authors:
Martin M Schulz; Maximilian G D Buschner; Richard Leidig; Heinz-D Wehner; Peter Fritz; Karina Häbig; Michael Bonin; Monika Schütz; Thomas Shiozawa; Frank Wehner
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Publication Detail:
Type:  Journal Article     Date:  2010-01-19
Journal Detail:
Title:  Journal of forensic sciences     Volume:  55     ISSN:  1556-4029     ISO Abbreviation:  J. Forensic Sci.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-16     Completed Date:  2010-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  492-8     Citation Subset:  IM    
Affiliation:
Institute of Legal Medicine, Eberhard-Karls-University of Tuebingen, Naegelestrasse 5, D-72074 Tuebingen, Germany. mail@dr-martin-schulz.de
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MeSH Terms
Descriptor/Qualifier:
Epidermis / cytology,  metabolism
Female
Forensic Pathology
Humans
Keratins / genetics,  metabolism*
Male
Mouth Mucosa / cytology,  metabolism
Penis / cytology,  metabolism
Polymerase Chain Reaction
RNA, Messenger / metabolism
Rectum / cytology,  metabolism
Sex Offenses*
Staining and Labeling
Vagina / cytology,  metabolism
Chemical
Reg. No./Substance:
0/RNA, Messenger; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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