| A new approach to the investigation of sexual offenses-cytoskeleton analysis reveals the origin of cells found on forensic swabs. | |
MedLine Citation:
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PMID: 20102457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There are forensic inquiries in which an identification of epithelial cell types would provide important probative evidence. In cancer diagnosis, this information is yielded by histological examination of cytokeratin (Ck). Therefore, we tested 19 antibodies against different Cks (Ck1, Ck2e, Ck4, Ck5-6, Ck7, Ck8, Ck9, CK10, Ck13, Ck14, Ck15, Ck16, Ck17, Ck18, Ck19, Ck20, Ck903, PanCkAE1_3, and CAM5-2) on histological sections of epidermis, buccal mucosa, vaginal mucosa, penis, urogenital tract, and rectum and could identify two antigens unique to buccal-cell and vaginal-cell (Ck4) and skin epithelial-cell (Ck10) cytokeratin. Subsequently, we developed an immunocytological technique for distinguishing swabbed skin and mucosal cells up to at least 1 year after sampling. By the detection of the Ck4 and Ck10 mRNAs in biopsy and laser capture microdissection collected samples via quantitative real-time polymerase chain reaction, we were able to confirm our immunological findings. Hence, this study offers techniques to discriminate between skin and mucosal cells (buccal and vaginal) in forensic casework. |
Authors:
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Martin M Schulz; Maximilian G D Buschner; Richard Leidig; Heinz-D Wehner; Peter Fritz; Karina Häbig; Michael Bonin; Monika Schütz; Thomas Shiozawa; Frank Wehner |
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Publication Detail:
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Type: Journal Article Date: 2010-01-19 |
Journal Detail:
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Title: Journal of forensic sciences Volume: 55 ISSN: 1556-4029 ISO Abbreviation: J. Forensic Sci. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-16 Completed Date: 2010-08-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375370 Medline TA: J Forensic Sci Country: United States |
Other Details:
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Languages: eng Pagination: 492-8 Citation Subset: IM |
Affiliation:
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Institute of Legal Medicine, Eberhard-Karls-University of Tuebingen, Naegelestrasse 5, D-72074 Tuebingen, Germany. mail@dr-martin-schulz.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Epidermis
/
cytology,
metabolism Female Forensic Pathology Humans Keratins / genetics, metabolism* Male Mouth Mucosa / cytology, metabolism Penis / cytology, metabolism Polymerase Chain Reaction RNA, Messenger / metabolism Rectum / cytology, metabolism Sex Offenses* Staining and Labeling Vagina / cytology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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