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A New Approach to Characterization of Insulin Derived from Different Species Using (1)H-NMR Coupled with Multivariate Analysis.
MedLine Citation:
PMID:  22382411     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Most of the active components of polypeptides have a complex molecular structure, large molecular size. Such components may also be structurally heterogeneous. Therefore, development of a method that can confirm the consistency of polypeptides amino-acid sequences for product characterization is desirable. In general, it is extremely difficult to distinguish differences of a few amino acid residues in the (1)H-NMR spectrum of polypeptides with molecular weights greater than several thousand. However, we have been able to distinguish between three insulin species differing in one to three amino acid residues using a combination of multivariate statistics and (1)H-NMR spectra. These results demonstrate that this methodology could be useful for characterization of polypeptides.
Authors:
Akiko Ohno; Toru Kawanishi; Haruhiro Okuda; Kiyoshi Fukuhara
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemical & pharmaceutical bulletin     Volume:  60     ISSN:  1347-5223     ISO Abbreviation:  Chem. Pharm. Bull.     Publication Date:  2012  
Date Detail:
Created Date:  2012-03-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0377775     Medline TA:  Chem Pharm Bull (Tokyo)     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  320-4     Citation Subset:  IM    
Affiliation:
Division of Organic Chemistry, National Institute of Health Sciences.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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