Document Detail

A New Approach for Measuring Single-Cell Oxygen Consumption Rates.
MedLine Citation:
PMID:  21057593     Owner:  NLM     Status:  Publisher    
A novel system that has enabled the measurement of single-cell oxygen consumption rates is presented. The experimental apparatus includes a temperature controlled environmental chamber, an array of microwells etched in glass, and a lid actuator used to seal cells in the microwells. Each microwell contains an oxygen sensitive platinum phosphor sensor used to monitor the cellular metabolic rates. Custom automation software controls the digital image data collection for oxygen sensor measurements, which are analyzed using an image-processing program to yield the oxygen concentration within each microwell versus time. Two proof-of-concept experiments produced oxygen consumption rate measurements for A549 human epithelial lung cancer cells of 5.39 and 5.27 fmol/min/cell, closely matching published oxygen consumption rates for bulk A549 populations.
Timothy W Molter; Sarah C McQuaide; Mark R Holl; Deirdre R Meldrum; Joseph M Dragavon; Judith B Anderson; A Cody Young; Lloyd W Burgess; Mary E Lidstrom
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Publication Detail:
Journal Detail:
Title:  IEEE transactions on automation science and engineering : a publication of the IEEE Robotics and Automation Society     Volume:  5     ISSN:  1558-3783     ISO Abbreviation:  -     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2010-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101225105     Medline TA:  IEEE Trans Autom Sci Eng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  32-42     Citation Subset:  -    
Department of Electrical Engineering, University of Washington, Seattle, WA 98195-2500 USA ( ; ).
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Grant Support
P50 HG002360-10//NHGRI NIH HHS

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