Document Detail

Neutrophils-induced increase of adenosine triphosphate depletion in rat neonatal cardiac myocytes with impaired energy metabolism.
MedLine Citation:
PMID:  1824299     Owner:  NLM     Status:  MEDLINE    
Isolated, cultured rat neonatal cardiac myocytes were placed in medium supplemented with mitochondrial respiratory inhibitor potassium cyanide which caused a rapid adenosine triphosphate (ATP) depletion. These myocytes with the impaired energy metabolism ("hypoxia-like state") were exposed to unstimulated human neutrophils. Effect of human neutrophils on the myocytes in the "hypoxia-like state" was quantified as a total change in the amount of ATP in cardiac cells. After 5 hours of incubation of neutrophils with the myocytes in the "hypoxia-like state" an additional decrease (of 50 per cent) in ATP content was observed. Since catalase (which destroys hydrogen peroxide) prevented the further decline in ATP level in the myocytes with impaired energy metabolism, it seem that hydrogen peroxide and possibly their products are responsible for this effect. These results suggest that unstimulated human neutrophils after activation by the contact with injured cardiac cells caused further decrease of ATP level in target cells.
M Gajewski; H Laskowska-Bozek; S Maśliński; J Ryzewski
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of physiology and pharmacology : an official journal of the Polish Physiological Society     Volume:  42     ISSN:  0867-5910     ISO Abbreviation:  J. Physiol. Pharmacol.     Publication Date:  1991 Dec 
Date Detail:
Created Date:  1992-10-16     Completed Date:  1992-10-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9114501     Medline TA:  J Physiol Pharmacol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  407-15     Citation Subset:  IM    
Department of Biochemistry, Institute of Rheumatology, Warsaw, Poland.
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MeSH Terms
Adenosine Triphosphate / analysis*
Anti-Inflammatory Agents, Non-Steroidal / pharmacology
Cells, Cultured
Energy Metabolism*
Myocardium / metabolism*
Neutrophils / physiology*
Rats, Inbred Strains
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 56-65-5/Adenosine Triphosphate

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