Document Detail

Neutrophils, but not lymphocytes or monocytes, infiltrate maternal systemic vasculature in women with preeclampsia.
MedLine Citation:
PMID:  19003640     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Leukocytes are activated in women with preeclampsia, but the class of leukocytes that infiltrates the maternal vasculature and, therefore, is most likely to cause vascular dysfunction is not known.
METHODS: Subcutaneous fat biopsies were obtained at Cesarean section or abdominal surgery from 7 normal non-pregnant women, 7 women with normal pregnancies, and 7 women with preeclampsia. Tissues were immunohistochemically stained for CD14, a monocyte/macrophage antigen, CD99, a lymphocyte antigen, and CD66b, a neutrophil antigen.
RESULTS: CD14 stained cells were found infiltrated into fat tissue but were not present in vessels for any of the groups. CD99-stained cells were present in approximately 20% to 30% of vessels with no difference among groups. CD66b-stained cells were present in all groups with a significantly greater percentage of vessels stained for preeclamptic than normal pregnant or normal non-pregnant women (70 +/- 6 vs. 43 +/- 9 vs. 21 +/- 5%, respectively, p < 0.01). CD66b cells were the most abundant cell type that infiltrated vessels of preeclamptic women.
CONCLUSIONS: 1) A significantly greater number of neutrophils adhered to endothelium and infiltrated into the intimal space in the maternal systemic vasculature of preeclamptic women than in that of normal pregnant women or normal non-pregnant women; 2) No significant difference in lymphocyte infiltration was observed among the patient groups, and lymphocytes were present in much lower numbers than-neutrophils; 3) Monocytes/macrophages were found in fat tissue but not in vessels. We speculate that neutrophils are the class of leukocytes that cause the majority of vascular cell dysfunction in women with preeclampsia.
Kristen A Cadden; Scott W Walsh
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Hypertension in pregnancy     Volume:  27     ISSN:  1525-6065     ISO Abbreviation:  Hypertens Pregnancy     Publication Date:  2008  
Date Detail:
Created Date:  2008-11-12     Completed Date:  2009-02-17     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  9421297     Medline TA:  Hypertens Pregnancy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  396-405     Citation Subset:  IM    
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MeSH Terms
Antigens, CD / analysis
Antigens, CD14 / analysis
Case-Control Studies
Cell Adhesion Molecules / analysis
Endothelium, Vascular / immunology*
GPI-Linked Proteins
Neutrophil Infiltration*
Pre-Eclampsia / immunology*
Subcutaneous Fat, Abdominal / blood supply,  immunology*
Young Adult
Grant Support
HL069851/HL/NHLBI NIH HHS; R01 HL069851/HL/NHLBI NIH HHS; R01 HL069851-03/HL/NHLBI NIH HHS; R01 HL069851-04/HL/NHLBI NIH HHS; R01 HL069851-05/HL/NHLBI NIH HHS
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD14; 0/CD99 protein, human; 0/CEACAM8 protein, human; 0/Cell Adhesion Molecules; 0/GPI-Linked Proteins

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