Document Detail

Neutrophil rheologic changes in septic shock.
MedLine Citation:
PMID:  9001286     Owner:  NLM     Status:  MEDLINE    
Decreases in effective capillary blood flow during septic shock may be related to changes in neutrophil rheology which contribute to microvascular occlusion. The purpose of this study was to examine neutrophil deformability, adherence, and aggregation in patients with severe sepsis and septic shock. Neutrophils were isolated from six patients with septic shock (SS), 12 patients with severe sepsis (S), six noninfected critically ill patients (CINS), and nine normal volunteers (N). Neutrophil deformability was determined by examining filtration through 5-microm filters. Neutrophil aggregation was measured by aggregometry and leukergy. Neutrophil adherence was examined by assessing the binding of latex beads to neutrophils. Patients with S and SS demonstrated decreased neutrophil filterability of 27 +/- 2% and 16 +/- 5%, respectively (p < 0.01), in comparison with N subjects, 55 +/- 4% and CINS patients, 58 +/- 2%. Preincubation of neutrophils from S and SS patients with cytochalasin D significantly increased the percent filtration of neutrophils. Neutrophil aggregation, measured by aggregometry, was increased in SS patients, 16 +/- 4% (p < 0.01) compared with N subjects, 1 +/- 0.2% and CINS patients, 1 +/- 0.2%. Incubation of neutrophils of SS patients with anti-CD11/CD18 significantly increased the filtration of isolated neutrophils to 46 +/- 3% (p < 0.01) and decreased aggregation to 7 +/- 2%. Neutrophil adherence was not increased in S or SS patients. These observations suggest that neutrophil deformability is decreased in patients with S and SS. Increased leukoaggregation may also contribute to decreased filterability of neutrophils in SS patients. These mechanisms may play a role in impaired microvascular flow in septic shock.
P C Yodice; M E Astiz; B M Kurian; R Y Lin; E C Rackow
Related Documents :
23506136 - Predictive value of lymphocytopenia and the neutrophil-lymphocyte count ratio for sever...
25021466 - Ptu-176 implementation of objective activity monitoring to supplement the interpretat...
24385726 - The value of serum immunoglobulin free light chain assessment in patients with monoclon...
23456496 - Cholecystectomy and clinical presentations of gastroparesis.
21231806 - A novel approach to measuring skin elasticity in systemic sclerosis: results from a pil...
23506136 - Predictive value of lymphocytopenia and the neutrophil-lymphocyte count ratio for sever...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  155     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-02-11     Completed Date:  1997-02-11     Revised Date:  2006-01-04    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  38-42     Citation Subset:  AIM; IM    
Department of Medicine, Saint Vincent's Hospital and Medical Center, New York, New York 10011, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Adhesion
Cell Aggregation
Neutrophils / physiology*
Sepsis / blood
Shock, Septic / blood*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Systemic, pulmonary and mesenteric perfusion and oxygenation effects of dopamine and epinephrine.
Next Document:  Higher concentrations of matrix metalloproteinases in bronchoalveolar lavage fluid of patients with ...