Document Detail

Neutrophil respiratory burst in term and preterm neonates without signs of infection and in those with increased levels of C-reactive protein.
MedLine Citation:
PMID:  8726239     Owner:  NLM     Status:  MEDLINE    
Developmental immaturities in neonatal host defense predispose the neonates to an increased mortality rate during bacterial infections. Early diagnosis is of great clinical importance, but, especially in neonates, is sometimes very difficult. The ability to generate reactive oxygen species, the so-called respiratory burst, is essential for neutrophils to kill infectious microorganisms. Therefore, changes of respiratory burst may reflect increased susceptibility of neonates to infections and may be useful for the early detection of infections. Superoxide anion production was determined by a flow cytometric method using dihydrorhodamine 123 (DHR) as an oxidative probe after priming of neutrophils with PBS buffer (spontaneous burst), with N-formyl-methionyl-leucyl-phenylalanine (fMLP), or with Escherichia coli. During the study period, the spontaneous percentage of activated cells in whole blood as well as the percentage of activated cells in stimulation with fMLP was lower in adults (n = 100; PBS, 1.0 +/- 0.1%; fMLP, 8.3 +/- 0.9%) compared with neonates without signs of infection (n = 143). Among the latter, the percentage of activated cells (PBS and fMLP assay) varied with respect to gestational age and hours of life: lowest values were measured in preterm newborns with gestational age less than 32 wk and between 25 and 120 h of life. The same correlation to gestational age was true for total neutrophil cell counts. In neonates with increased levels of C-reactive protein during the first 5 d of life (n = 43), the percentages of activated cells after PBS and fMLP incubation were higher than those of neonates without signs of infection. The relationship of neutrophil respiratory burst and neutrophil cell counts to gestational age might reflect at least in part a reason for the increased susceptibility of neonates to infections. Furthermore, determination of respiratory burst may prove to be a new laboratory parameter of neonatal infection.
P Gessler; T Nebe; A Birle; N Haas; W Kachel
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pediatric research     Volume:  39     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-10-21     Completed Date:  1996-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  843-8     Citation Subset:  IM    
Department of Pediatrics, Universitaets-Kinderklinik, Mannheim, Germany.
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MeSH Terms
Bacterial Infections / blood,  etiology,  immunology
Blood Bactericidal Activity / drug effects
C-Reactive Protein / metabolism*
Escherichia coli / immunology
Infant, Newborn / blood*,  immunology
Infant, Premature / blood*,  immunology
Leukocyte Count
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
Neutrophils / drug effects,  immunology,  metabolism*
Respiratory Burst* / drug effects
Reg. No./Substance:
59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 9007-41-4/C-Reactive Protein

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