Document Detail


Neutrophil-related immunoinflammatory disturbance in steroid-overdosed ulcerative colitis patients.
MedLine Citation:
PMID:  18958548     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: There is some evidence that large preoperative doses of steroids are a causative factor for postoperative higher morbidity in ulcerative colitis (UC) patients. This study aimed to assess steroid-related changes in functional profiles of neutrophils in UC patients to estimate the immunological changes under surgical stress. METHODS: Neutrophils were extracted from peripheral blood of 30 UC patients and 30 healthy controls. UC patients whose neutrophils were isolated were divided into two subgroups according to their total preoperative dosage of prednisolone: group H, > or =10,000 mg; group L, <10,000 mg. Expression of neutrophil surface antigens was analyzed and neutrophil phagocytosis was evaluated. Patterns of cell death of neutrophils were evaluated by co-culturing with Escherichia coli. Production of inflammatory mediators in cultured neutrophils was assessed. RESULTS: There were no significant differences in the expression rates of TLR4, CD11b, and CD16b on neutrophils (CD15(+) cells) between the two patient groups and controls. There was also no significant difference in neutrophil phagocytosis between the two patient groups and controls. The neutrophil necrosis rate in group H was higher than that in group L and the controls 3 h after exposure to E. coli. Neutrophils from group H released the highest levels of proinflammatory cytokines following interleukin-1beta or lipopolysaccharide stimulation. Neutrophils from group H also released the highest levels of proteolytic enzymes. CONCLUSIONS: Steroid-overdosed UC patients may have a functional deficit in neutrophils, which may cause a postsurgical systemic "storm" of inflammatory mediators.
Authors:
Shigeyuki Yoshiyama; Chikao Miki; Yoshiki Okita; Toshimitsu Araki; Keiichi Uchida; Masato Kusunoki
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Publication Detail:
Type:  Controlled Clinical Trial; Journal Article     Date:  2008-10-29
Journal Detail:
Title:  Journal of gastroenterology     Volume:  43     ISSN:  0944-1174     ISO Abbreviation:  J. Gastroenterol.     Publication Date:  2008  
Date Detail:
Created Date:  2008-10-29     Completed Date:  2009-02-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430794     Medline TA:  J Gastroenterol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  789-97     Citation Subset:  IM    
Affiliation:
Department of Gastrointestinal Surgery, Division of Reparative Medicine, Institute of Life Sciences, Mie University Graduate School of Medicine, 2-174 Edobashi, Tsu 514-8507, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Apoptosis / physiology
Cohort Studies
Colitis, Ulcerative / drug therapy*,  immunology*,  surgery
Cytokines / metabolism
Dose-Response Relationship, Drug
Female
Glucocorticoids / administration & dosage*
Humans
Male
Membrane Proteins / metabolism
Neutrophil Activation / physiology*
Neutrophils / physiology*
Phagocytosis / physiology
Prednisolone / administration & dosage*
Chemical
Reg. No./Substance:
0/Cytokines; 0/Glucocorticoids; 0/Membrane Proteins; 50-24-8/Prednisolone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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