Document Detail

Neutrophil primary granule proteins HBP and HNP1-3 boost bacterial phagocytosis by human and murine macrophages.
MedLine Citation:
PMID:  18787642     Owner:  NLM     Status:  MEDLINE    
In acute inflammation, infiltrating polymorphonuclear leukocytes (also known as PMNs) release preformed granule proteins having multitudinous effects on the surrounding environment. Here we present what we believe to be a novel role for PMN-derived proteins in bacterial phagocytosis by both human and murine macrophages. Exposure of macrophages to PMN secretion markedly enhanced phagocytosis of IgG-opsonized Staphylococcus aureus both in vitro and in murine models in vivo. PMN secretion activated macrophages, resulting in upregulation of the Fcgamma receptors CD32 and CD64, which then mediated the enhanced phagocytosis of IgG-opsonized bacteria. The phagocytosis-stimulating activity within the PMN secretion was found to be due to proteins released from PMN primary granules; thorough investigation revealed heparin-binding protein (HBP) and human neutrophil peptides 1-3 (HNP1-3) as the mediators of the macrophage response to PMN secretion. The use of blocking antibodies and knockout mice revealed that HBP acts via beta2 integrins, but the receptor for HNP1-3 remained unclear. Mechanistically, HBP and HNP1-3 triggered macrophage release of TNF-alpha and IFN-gamma, which acted in an autocrine loop to enhance expression of CD32 and CD64 and thereby enhance phagocytosis. Thus, we attribute what may be a novel role for PMN granule proteins in regulating the immune response to bacterial infections.
Oliver Soehnlein; Ylva Kai-Larsen; Robert Frithiof; Ole E Sorensen; Ellinor Kenne; Karin Scharffetter-Kochanek; Einar E Eriksson; Heiko Herwald; Birgitta Agerberth; Lennart Lindbom
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  118     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-02     Completed Date:  2008-11-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3491-502     Citation Subset:  AIM; IM    
Department of Physiology and Pharmacology and Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
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MeSH Terms
Bacteria / immunology*
Cells, Cultured
Disease Models, Animal
Gene Expression Regulation
Interferon-gamma / metabolism
LDL-Receptor Related Protein-Associated Protein / metabolism*
Macrophages / cytology,  immunology*,  microbiology*
Macrophages, Peritoneal / immunology,  microbiology
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Neutrophils / secretion*
Peritonitis / physiopathology
Receptors, IgG / metabolism
Signal Transduction
Tumor Necrosis Factor-alpha / metabolism
alpha-Defensins / metabolism*
Reg. No./Substance:
0/FCGR1A protein, human; 0/LDL-Receptor Related Protein-Associated Protein; 0/Receptors, IgG; 0/Tumor Necrosis Factor-alpha; 0/alpha-Defensins; 82115-62-6/Interferon-gamma

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