| Neutrophil oxidative metabolism in Down syndrome patients with congenital heart defects. | |
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MedLine Citation:
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PMID: 19593803 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Down syndrome (DS) occurs when an individual has three, rather than two, copies of the 21st chromosome. Cytosolic superoxide dismutase (SOD-1) is encoded by a gene on chromosome 21 and thus, SOD-1 activity is elevated in patients with DS. Forty percent of all cases with DS are associated with congenital heart defects (CHD). Although the contribution of SOD1 to disease phenotype is unknown, it is considered to be a "molecular marker" of the disease. It was hypothesized herein that the presence of CHD may alter the expression of SOD1 and oxidative metabolism in patients with DS. This hypothesis was tested via four experimental groups as follows: patients with DS without CHD, DS patients with CHD, CHD patients without DS and controls. Expression and activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), myeloperoxidase (MPO), and catalase (CAT) were determined in neutrophils from all experimental groups. Intracellular hydrogen peroxide concentration and superoxide release were also evaluated in neutrophils. A significant increase was observed in SOD and GPx amount and activity in patients with DS with and without CHD. No significant difference was found in the amount and activity of MPO and CAT among the different experimental groups. Intracellular hydrogen peroxide concentration was similar in all groups, whereas a prominent decrease was seen in superoxide release in cases with DS. Patients with DS with and without CHD showed no significant differences in any of the measured parameters. The data suggest that CHD observed in patients with DS does not result from altered redox metabolism associated with the disease. |
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Authors:
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Ozlem Akinci; Ercan Mihci; Sukran Tacoy; Firat Kardelen; Ibrahim Keser; Mutay Aslan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Environmental and molecular mutagenesis Volume: 51 ISSN: 1098-2280 ISO Abbreviation: Environ. Mol. Mutagen. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-11 Completed Date: 2010-01-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8800109 Medline TA: Environ Mol Mutagen Country: United States |
Other Details:
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Languages: eng Pagination: 57-63 Citation Subset: IM |
Copyright Information:
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(c) 2009 Wiley-Liss, Inc. |
Affiliation:
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Department of Pediatric Genetics, Akdeniz University School of Medicine, Antalya, Turkey. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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metabolism Blotting, Western Catalase / genetics, metabolism Child Down Syndrome / complications*, enzymology*, genetics Female Glutathione Peroxidase / genetics, metabolism Heart Defects, Congenital / etiology*, genetics Humans Hydrogen Peroxide / chemistry Male Neutrophils / chemistry, metabolism* Oxidation-Reduction Superoxide Dismutase / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.6/Catalase; EC 1.11.1.9/Glutathione Peroxidase; EC 1.15.1.1/Superoxide Dismutase |
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