Document Detail


Neutrophil gelatinase-associated lipocalin acts as a protective factor against H(2)O(2) toxicity.
MedLine Citation:
PMID:  18662586     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Lipocalin 2 (Lcn2, NGAL) is a member of the lipocalin superfamily for which a variety of functions have been reported. However, the precise biological roles of NGAL are not fully known. We have investigated the ability of NGAL to prevent H(2)O(2) toxicity, which is considered to be the classical inducer of oxidative stress caused by ROS generation in an in vitro model. METHODS: NGAL cDNA was isolated from HepG2 cell line and cloned to pcDNA3.1(+) vector. The construct was transfected to CHO cell line. Stable clones were generated, and the expression of NGAL was determined by RT-PCR, Western blot analysis and ELISA. NGAL gene in A549 cell line was downregulated with the siRNA. CHO and A549 cells were intoxicated with H(2)O(2) and cell proliferation was performed by MTT assay. Apoptotic cells were detected by flow cytometry. RESULTS: Cell proliferation was higher in CHO expressing NGAL in doses of 5 and 10 mM H(2)O(2) after 2h compared with the control. H(2)O(2) was also more toxic in the presence of NGAL siRNA compared with the control in A549 cell. Our results also revealed that NGAL protect cells from apoptosis. CONCLUSIONS: Overall, our results revealed for the first time a new function for NGAL/Lcn2: acting as a protective factor against H(2)O(2) toxicity. In the future, NGAL may have the potential application to ameliorate the toxicity induced by oxidative stress conditions.
Authors:
Mehryar Habibi Roudkenar; Raheleh Halabian; Zahra Ghasemipour; Amaneh Mohammadi Roushandeh; Mahdi Rouhbakhsh; Mahin Nekogoftar; Yoshikazu Kuwahara; Manabu Fukumoto; Mohammad Ali Shokrgozar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of medical research     Volume:  39     ISSN:  0188-4409     ISO Abbreviation:  Arch. Med. Res.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-29     Completed Date:  2008-09-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9312706     Medline TA:  Arch Med Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  560-6     Citation Subset:  IM    
Affiliation:
Research Center, Iranian Blood Transfusion Organization, Tehran Iran. Roudkenar@ibto.ir
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MeSH Terms
Descriptor/Qualifier:
Acute-Phase Proteins / genetics,  metabolism*
Animals
Apoptosis
Base Sequence
CHO Cells
Cell Line
Cricetinae
Cricetulus
DNA Primers
Humans
Hydrogen Peroxide / pharmacology*
Lipocalins / genetics,  metabolism*
Proto-Oncogene Proteins / genetics,  metabolism*
RNA, Small Interfering
Chemical
Reg. No./Substance:
0/Acute-Phase Proteins; 0/DNA Primers; 0/LCN2 protein, human; 0/Lipocalins; 0/Proto-Oncogene Proteins; 0/RNA, Small Interfering; 7722-84-1/Hydrogen Peroxide

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