| Neutrophil elastase and its complex with alpha 1-antitrypsin in soluble and insoluble fractions of nasal secretions of chronic sinusitis. | |
| | |
MedLine Citation:
|
PMID: 1759584 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Immunoreactive neutrophil elastase (NE) and its complex with alpha 1-antitrypsin (AT) was measured by double antibody enzyme linked immunosorbent assay (ELISA) in nasal secretions of chronic sinusitis (CS). Nasal secretions were separated into two fractions: PBS-soluble and insoluble fractions. Elastolytic activity was also examined. Mean value of total NE level was 31.0 micrograms/ml in the soluble fraction, which was significantly lower than that in the insoluble fraction (71.9 micrograms/ml, p less than 0.01). On the other hand, the percentage of complexed NE in total NE in the soluble fraction (33.7%) was significantly higher than that in the insoluble fraction (12.1%, p less than 0.01). Elastolytic activity in the soluble fraction (23.4 RFU) was significantly lower than that in the insoluble fraction (170.5 RFU, p less than 0.01). NE with elastolytic activity exists in nasal secretions of CS, and active-free NE in the insoluble fraction could be a major source of enhancement and continuation of mucosal inflammation. |
| | |
Authors:
|
Y Hamaguchi; H Suzumura; C S Jin; Y Sakakura |
Related Documents
:
|
14744744 - Delivery of tea polyphenols to the oral cavity by green tea leaves and black tea extract. 6613544 - The effects of camphor, eucalyptus and menthol vapour on nasal resistance to airflow an... 6309134 - Flavor-illness aversions: potentiation of odor by taste is disrupted by application of ... 2044504 - A comparison of techniques to enhance the evaluation of equine laryngeal function. 16018344 - Saccadic velocity and pupillary reflexes during acclimatization to altitude (4300 m). 19124984 - Pilot study of a 10-week multidisciplinary tai chi intervention in sedentary obese women. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Acta oto-laryngologica Volume: 111 ISSN: 0001-6489 ISO Abbreviation: Acta Otolaryngol. Publication Date: 1991 |
Date Detail:
|
Created Date: 1992-02-03 Completed Date: 1992-02-03 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370354 Medline TA: Acta Otolaryngol Country: SWEDEN |
Other Details:
|
Languages: eng Pagination: 954-9 Citation Subset: IM |
Affiliation:
|
Department of Otorhinolaryngology, Mie University School of Medicine, Tsu, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Chronic Disease Enzyme-Linked Immunosorbent Assay Female Humans Immunoenzyme Techniques Male Molecular Sequence Data Nasal Mucosa / enzymology*, secretion Neutrophils / enzymology* Pancreatic Elastase / chemistry, metabolism* Sinusitis / enzymology* Solubility alpha 1-Antitrypsin / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/alpha 1-Antitrypsin; EC 3.4.21.36/Pancreatic Elastase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Macrophages on the nasal mucosal surface in provoked and naturally occurring allergic rhinitis.
Next Document: The effect of pilocarpine and atropine administration on radiation-induced injury of rat submandibul...