| Neutrophil dysfunction after biomaterial contact in an in vitro model of cardiopulmonary bypass. | |
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MedLine Citation:
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PMID: 17029835 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Cardiopulmonary bypass (CPB) induces neutrophil degranulation and superoxide anion production in vivo. We hypothesized that CPB-associated neutrophil dysregulation alters neutrophil adhesion to vascular endothelial cells and the extracellular matrix. METHODS: We, therefore, recirculated neutrophils in polyvinyl chloride (PVC) tubing using a roller pump model and thereafter measured adhesion to cultured microvascular endothelial cells and gelatin-coated surfaces. Recirculation-induced neutrophil priming or exhaustion was tested by boosting with phorbol myristate-acetate (PMA) or N-formyl-methiolyl-leucyl-phenylalanine (FMLP) before quantification of adhesion. RESULTS AND CONCLUSION: After recirculation, neutrophils retained their adhesive capability to vascular endothelial cells, whereas adhesion to gelatin increased. This increase was not seen when the neutrophils were recirculated with a rotator instead of a roller pump, indicating that not only the pump mode but also foreign surface contact was of significance. The neutrophil PMA response after recirculation was not altered compared to resting neutrophils prestimulated with PMA. Recirculated neutrophils adhered less to cultured vascular endothelial cells after FMLP activation and more to gelatin compared to resting neutrophils prestimulated with FMLP. It is conceivable that dysregulation of neutrophil adhesive capability may play a part in the development of tissue damage after CPB. |
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Authors:
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Ann E Asberg; Vibeke Videm |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-06 |
Journal Detail:
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Title: European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery Volume: 30 ISSN: 1010-7940 ISO Abbreviation: Eur J Cardiothorac Surg Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-10-23 Completed Date: 2007-03-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8804069 Medline TA: Eur J Cardiothorac Surg Country: Germany |
Other Details:
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Languages: eng Pagination: 744-8 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine, Children's and Women's Health, Norwegian University of Science and Technology, Trondheim, Norway. ann.asberg@ntnu.no |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials* Cardiopulmonary Bypass* Cell Adhesion / physiology Cells, Cultured Endothelium, Vascular / cytology Gelatin Humans Models, Cardiovascular N-Formylmethionine Leucyl-Phenylalanine / pharmacology Neutrophil Activation / physiology Neutrophils / drug effects, physiology* Polyvinyl Chloride Surface Properties Tetradecanoylphorbol Acetate / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 16561-29-8/Tetradecanoylphorbol Acetate; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 9000-70-8/Gelatin; 9002-86-2/Polyvinyl Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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