| Neutrophil-degranulating action of 5,12-dihydroxy-6,8,10,14-eicosatetraenoic acid and 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine. Comparison with other degranulating agents. | |
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MedLine Citation:
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PMID: 6274199 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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5,12-Dihydroxy-6,8,10,14-eicosatetraenoic acid was prepared from rabbit neutrophils challenged with ionophore A23187 plus arachidonic acid. It was purified by reverse-phase high-performance liquid chromatography with the use of a two-step procedure that effectively resolved the lipid from closely eluting contaminants. The phospholipid 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine was prepared from beef heart plasmalogen. Confirming earlier reports, both lipids stimulated human polymorphonuclear neutrophils to release granule-bound enzymes. Their respective degranulating actions took less than 5 minutes to become maximal, were dependent upon the presence of cytochalasin B, and required intact pathways of arachidonic acid metabolism, as judged by the inhibitory actions of arachidonate antimetabolites. Neither lipid required more than trace concentrations of extracellular calcium to effect optimal amounts of enzyme release. In one or more of these regards the two lipids differed strikingly from other neutrophil-degranulating agents such as A23187, N-formyl-methionyl-leucyl-phenylalanine, and phorbol myristate acetate. The mechanism of action of the two lipid stimuli, therefore, appear to differ somewhat from the mechanisms of the other three stimuli. |
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Authors:
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J T O'Flaherty; R L Wykle; C J Lees; T Shewmake; C E McCall; M J Thomas |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of pathology Volume: 105 ISSN: 0002-9440 ISO Abbreviation: Am. J. Pathol. Publication Date: 1981 Dec |
Date Detail:
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Created Date: 1982-02-12 Completed Date: 1982-02-12 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 264-9 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid Arachidonic Acids / metabolism, pharmacology* Calcimycin / pharmacology Calcium / metabolism Cattle Chemotactic Factors / pharmacology Cytochalasin B / pharmacology Cytoplasmic Granules / drug effects* Humans Leukotriene B4 Lysophosphatidylcholines / pharmacology* N-Formylmethionine / analogs & derivatives, pharmacology N-Formylmethionine Leucyl-Phenylalanine Neutrophils / drug effects* Oligopeptides / pharmacology Platelet Activating Factor Rabbits Stimulation, Chemical Tetradecanoylphorbol Acetate / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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AI-09169/AI/NIAID NIH HHS; HL-26257/HL/NHLBI NIH HHS; HL-27799/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Chemotactic Factors; 0/Lysophosphatidylcholines; 0/Oligopeptides; 0/Platelet Activating Factor; 14930-96-2/Cytochalasin B; 16561-29-8/Tetradecanoylphorbol Acetate; 4289-98-9/N-Formylmethionine; 506-32-1/Arachidonic Acid; 52665-69-7/Calcimycin; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine; 71160-24-2/Leukotriene B4; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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