Document Detail

Neutrophil aggregating and chemokinetic properties of a 5,12,20-trihydroxy-6,8,10,14-eicosatetraenoic acid isolated from human leukocytes.
MedLine Citation:
PMID:  6289379     Owner:  NLM     Status:  MEDLINE    
Suspensions of human peripheral blood leukocytes were incubated with arachidonic acid and calcium ionophore A23187. Acidic lipid extracts were purified by silicic acid chromatography and reversed phase high performance liquid chromatography. A polar metabolite with an ultraviolet absorption spectrum identical to that of leukotriene B4 was identified by gas chromatography and mass spectrometry as a 5,12,20-trihydroxy-6,8,10,14-eicosatetraenoic acid. The data indicated that this compound was the 20-hydroxy metabolite of leukotriene B4. The compound showed chemokinetic activity towards human polymorphonuclear leukocytes and aggregating activity towards rat peritoneal polymorphonuclear leukocytes. Its activity was greater than that of 5-hydroxy-6,8,11,14-eicosatetraenoic acid but less than that of leukotriene B4. It is concluded that the 15,12,20-trihydroxyeicosatetraenoic acid may play a role as a mediator of inflammatory reactions.
R D Camp; P M Woollard; A I Mallet; N J Fincham; A W Ford-Hutchinson; M A Bray
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prostaglandins     Volume:  23     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  1982 May 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  631-41     Citation Subset:  IM    
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MeSH Terms
Calcimycin / pharmacology
Chemotaxis, Leukocyte / drug effects*
Chromatography, High Pressure Liquid
Gas Chromatography-Mass Spectrometry
Leukocytes / analysis*,  drug effects
Leukotriene B4 / analogs & derivatives*,  blood,  pharmacology
Neutrophils / drug effects*
Reg. No./Substance:
52665-69-7/Calcimycin; 71160-24-2/Leukotriene B4; 79008-24-5/5,12,20-trihydroxy-6,8,10,14-eicosatetraenoic acid

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