Document Detail


Neutrophil adhesion molecules in term and premature infants: normal or enhanced leucocyte integrins but defective L-selectin expression and shedding.
MedLine Citation:
PMID:  7542575     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The functional deficits of neonatal neutrophils are well documented and are thought to contribute to the increased susceptibility of newborn infants to infection. We measured the adhesion molecules L-selectin, CD11a/CD18 and CD11b/CD18 on neutrophils from the cord blood of term (n = 22) and premature (n = 32) infants using a whole blood method with flow cytometry and quantitative bead standards to enumerate cell surface receptors. We also assayed plasma for the shed form of L-selectin (sL-selectin). Our results suggested that L-selectin expression on term infant neutrophils is lower than that on adult neutrophils (unstimulated and stimulated, both P < 0.001), but that stimulated premature infant cell express higher L-selectin than term infants (P < 0.05); it is possible that this deficiency is caused by physiological changes occurring around the normal time of parturition. We observed reduced sL-selectin in term infants (P < 0.001) compared with adults, and even lower concentrations in premature infants (P < 0.001). The sL-selectin concentrations in plasma may be a reflection of granulopoiesis, which may be reduced in premature infants. Our results showed increased resting neonatal neutrophil expression of CD11b/CD18 compared with adults, and the absence of any neonatal deficit of the ability to up-regulate CD11b/CD18 expression on stimulation. These findings are contrary to previous reports. Further studies suggested that the isolation procedures used in previous reports reduces the capability of the cells to respond to a formyl methionine leucine phenylalanine (fMLP) stimulus. This effect is more marked in neonatal neutrophils, suggesting that the previously reported deficiency is in fact due to the isolation techniques used rather than the cells' innate ability to up-regulate CD11b/CD18 expression. The results of our study lead us to propose that the adhesive function of neonatal neutrophils may be less defective than previously thought.
Authors:
N Rebuck; A Gibson; A Finn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  101     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-08-30     Completed Date:  1995-08-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  183-9     Citation Subset:  IM    
Affiliation:
Department of Paediatrics, University of Sheffield, Children's Hospital, UK.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD11 / biosynthesis
Antigens, CD18 / biosynthesis
Cell Adhesion
Cell Adhesion Molecules / biosynthesis*
Cell Separation / methods
Humans
Infant, Newborn
Infant, Premature / blood*
Integrins / biosynthesis*
L-Selectin
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
Neutrophils / chemistry*,  drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Antigens, CD11; 0/Antigens, CD18; 0/Cell Adhesion Molecules; 0/Integrins; 126880-86-2/L-Selectin; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine
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