Document Detail

Neutrophil ablation with anti-serum does not protect against phase 2 ventricular arrhythmias in anaesthetised rats with myocardial infarction.
MedLine Citation:
PMID:  17261276     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: Arrhythmias, including ventricular fibrillation (VF), occur in two phases after coronary obstruction, the first during the reversible stage of acute myocardial ischaemia (phase 1) and the second during evolution of the infarct (phase 2). We tested the hypothesis that phase 2 arrhythmias are mediated by actions of neutrophils accumulating within the infarct. METHODS: Male rats (n=10 per group) were randomized to receive 2 ml/kg i.p. of either rabbit anti-rat neutrophil anti-serum or normal rabbit serum. After 17 h, single stage left coronary artery ligation was performed under pentobarbitone anaesthesia, and ischaemia was maintained for 240 min. RESULTS: Anti-serum pretreatment caused almost total neutropenia, reducing neutrophils in circulating blood from 2096+/-274x10(3) to 8+/-8x10(3) per ml (p<0.05). It also blocked neutrophil accumulation in the infarct, reducing cardiac myeloperoxidase activity from 74.7+/-27.4 to 9+/-3 mU per mg protein (p<0.05). Despite this, there was no significant difference between control and anti-serum-treated rats in the incidence of phase 2 VF (30% in each group) tachycardia (VT; 60% vs 80%) or number of ventricular premature beats (VPBs). CONCLUSION: Neutrophil accumulation within the evolving myocardial infarct does not mediate phase 2 VF.
Hugh Clements-Jewery; David J Hearse; Michael J Curtis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-23
Journal Detail:
Title:  Cardiovascular research     Volume:  73     ISSN:  0008-6363     ISO Abbreviation:  Cardiovasc. Res.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-19     Completed Date:  2007-05-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077427     Medline TA:  Cardiovasc Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  761-9     Citation Subset:  IM    
Loyola University Medical Center, Maywood, IL, USA.
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MeSH Terms
Immune Sera / pharmacology*
Models, Animal
Myocardial Infarction / immunology*,  pathology
Myocardium / metabolism,  pathology
Neutrophils / immunology,  physiology*
Peroxidase / analysis
Rats, Sprague-Dawley
Ventricular Fibrillation / immunology*
Ventricular Premature Complexes
Reg. No./Substance:
0/Immune Sera; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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