| Neutrophil IL-10 suppresses peritoneal inflammatory monocytes during polymicrobial sepsis. | |
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MedLine Citation:
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PMID: 21106642 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Septic peritonitis remains a major cause of death. Neutrophils and inflammatory monocytes are principal components of the innate immune system and are essential for defense against a range of microbial pathogens. Their role and interaction in polymicrobial sepsis have not been defined clearly. Using a murine model of CLP to induce moderate sepsis, we found that neutrophil depletion did not alter survival, whereas depletion of neutrophils and inflammatory monocytes markedly reduced survival. After neutrophil depletion, inflammatory monocytes had greater phagocytic capacity and oxidative burst, and increased expression of costimulatory molecules, TNF, and iNOS. Notably, peritoneal neutrophils produced IL-10 following CLP. Adoptive i.p. transfer of WT but not IL-10(-/-) neutrophils into septic mice reduced monocyte expression of TNF. In vitro experiments confirmed that monocyte suppression was mediated by neutrophil-derived IL-10. Thus, during septic peritonitis, neutrophils suppress peritoneal inflammatory monocytes through IL-10 and are dispensable for survival. |
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Authors:
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Lee M Ocuin; Zubin M Bamboat; Vinod P Balachandran; Michael J Cavnar; Hebroon Obaid; George Plitas; Ronald P DeMatteo |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-11-24 |
Journal Detail:
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Title: Journal of leukocyte biology Volume: 89 ISSN: 1938-3673 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-01 Completed Date: 2011-04-20 Revised Date: 2012-03-01 |
Medline Journal Info:
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Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: United States |
Other Details:
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Languages: eng Pagination: 423-32 Citation Subset: IM |
Affiliation:
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Hepatopancreatobiliary Service, Memorial Sloan-Kettering Cancer Center, New York, New York, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Inflammation / complications, immunology, pathology Interleukin-10 / immunology* Male Mice Mice, Inbred C57BL Monocytes / enzymology, pathology* Neutrophils / enzymology, immunology* Nitric Oxide Synthase Type II / metabolism Peritonitis / complications*, immunology*, pathology Sepsis / complications, immunology*, microbiology*, pathology Survival Analysis Tumor Necrosis Factor-alpha / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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AI70658/AI/NIAID NIH HHS; DK068346/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Tumor Necrosis Factor-alpha; 130068-27-8/Interleukin-10; EC 1.14.13.39/Nitric Oxide Synthase Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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