Document Detail


Neutralizing and enhancing activities of human respiratory syncytial virus-specific antibodies.
MedLine Citation:
PMID:  8705669     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The neutralizing and enhancing activities of respiratory syncytial virus (RSV)-specific antibodies were examined. These two biological activities were measured for a panel of six monoclonal antibodies (MAbs) specific to the RSV surface F and G glycoproteins. Four MAbs specific for the F protein possessed both neutralizing and enhancing activities. One MAb (11-2-D2), specific to the G protein, enhanced RSV infection of U937 cells, a human macrophage cell line, but did not neutralize virus infectivity. One MAb (11-3-A3), specific to the F protein, efficiently neutralized virus infectivity but did not enhance RSV infection of U937 cells. MAb 11-3-A3 neutralized representative strains of the two antigenic subtypes of RSV. Assays performed with mixtures of MAbs showed that high concentrations of MAb 11-3-A3 masked the enhancing activity of MAb 11-2-D2. The assay of mixtures of two MAbs possessing only enhancing activities demonstrated that this response was synergistic. The role of neutralizing and enhancing antibodies in determining the outcome of RSV infection was examined for infants from whom cord blood serum samples were collected at birth. There was no significant difference in the magnitude of the serum-enhancing activities between infants who were hospitalized with RSV infections and a group of age- and sex-matched control infants with no reported respiratory illness requiring hospitalization. However, the results indicated a possible correlation between RSV infection of the infants and the occurrence of in vitro antibody-dependent enhancement of the cord blood sera at a serum dilution of 10(-2). A significant inverse correlation was found between the plaque-neutralizing and enhancing activities of the cord blood sera from infants, irrespective of subsequent RSV infection. These data are discussed in relation to the possible contribution of antibody-dependent enhancement to the normal course of RSV pathology in vivo.
Authors:
H B Gimenez; S Chisholm; J Dornan; P Cash
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and diagnostic laboratory immunology     Volume:  3     ISSN:  1071-412X     ISO Abbreviation:  Clin. Diagn. Lab. Immunol.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-09-06     Completed Date:  1996-09-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9421292     Medline TA:  Clin Diagn Lab Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  280-6     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, Scotland.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / chemistry
Antibodies, Viral / analysis,  immunology*
Antibody Specificity
Antibody-Dependent Enhancement
Fetal Blood / immunology
Humans
Infant, Newborn
Neutralization Tests
Respiratory Syncytial Virus, Human / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral
Comments/Corrections

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