Document Detail

Neutralization of maternal IL-4 modulates congenital protozoal transmission: comparison of innate versus acquired immune responses.
MedLine Citation:
PMID:  10779783     Owner:  NLM     Status:  MEDLINE    
IL-4 levels were modulated in mice to test the hypothesis that induction of a maternal type 1 response would decrease the frequency of congenital Neospora caninum transmission. This hypothesis tested the relationship between IL-4 and both innate and adaptive immunity utilizing two basic experimental designs. In the first, maternal IL-4 was neutralized with mAb during pregnancy in naive mice concomitant with initial, virulent infection. In the second, maternal IL-4 was neutralized before pregnancy concomitant with a priming inoculation consisting of live, avirulent N. caninum tachyzoites followed by virulent challenge during subsequent gestation. In mice that were naive before pregnancy, neutralization of IL-4 during gestational challenge did not result in decreased congenital transmission as measured by PCR performed on 1-day-old neonatal mice. In mice that were primed and modulated before pregnancy, congenital transmission from gestational challenge was significantly decreased compared with control mice. Reduction in transmission constituted a decrease in the numbers of mice transmitting N. caninum and a lower frequency of transmission by individual dams (p < 0.05). Decreased congenital transmission was associated with significantly lower levels of maternal splenocyte IL-4 secretion, lower IL-4 mRNA levels, and higher levels of IFN-gamma secretion. Protected mice had significantly decreased Neospora-specific IgG1 compared with nonmodulated mice. These studies define a relationship between maternal Ag-specific immunity and the frequency of congenital transmission and demonstrate that modulation of type 2 cytokine responses can change the frequency of congenital protozoal transmission.
M T Long; T V Baszler
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  164     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-05-22     Completed Date:  2000-05-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4768-74     Citation Subset:  AIM; IM    
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610, USA.
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MeSH Terms
Antibodies, Monoclonal / pharmacology
Antigens, Protozoan / administration & dosage,  immunology
Coccidiosis / congenital,  immunology*,  transmission*
Enzyme-Linked Immunosorbent Assay
Immunity, Innate
Immunity, Maternally-Acquired*
Immunoglobulin G / biosynthesis
Interferon-gamma / genetics,  metabolism,  secretion
Interleukin-4 / immunology*,  metabolism*,  secretion
Mice, Inbred BALB C
Neospora / immunology*
RNA, Messenger / metabolism
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Protozoan; 0/Immunoglobulin G; 0/RNA, Messenger; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma

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