Document Detail


Neutralization of interleukin-1beta reduces vasospasm and alters cerebral blood vessel density following experimental subarachnoid hemorrhage in rats.
MedLine Citation:
PMID:  19442158     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Subarachnoid hemorrhage (SAH) develops when extravasated arterial blood enters subarachnoid space and mixes with cerebrospinal fluid. As a result, many pathologies develop, including arterial vasospasm that leads to the ischemia and hypoxia. Immuno-inflammatory response is considered as the cause of numerous complications following SAH. In the study, we examined the role of one of major cytokines, interleukin 1-beta (IL-1beta), on the vascular pathologies after experimental SAH in adult rats. SAH was produced by injection of 150 uL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1beta activity was inhibited by intracerebroventricular administration of anti-rat IL-1beta antibodies (SAH' groups). Control group consisted of sham-operated rats. Ninety minutes or 24 hrs following surgery, animals were perfused transcardially and whole brains were collected. Spasm index (ratio of vessel diameter to the mean wall thickness) of basilar artery as well as blood vessel density (number of vessels per square millimeter) at brain stem and frontal part of the brain were measured. SAH led to the vasospasm of basilar artery and increased the density of blood vessel. Neutralization of IL-1beta activity significantly reduced both the vasospasm and blood vessel density only 24 hrs after SAH. The results demonstrate an important role of IL-1beta in the delayed development of vascular pathologies after subarachnoid hemorrhage.
Authors:
Halina Jedrzejowska-Szypułka; Magdalena Larysz-Brysz; Michał Kukla; Mirosław Snietura; Joanna Lewin-Kowalik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current neurovascular research     Volume:  6     ISSN:  1875-5739     ISO Abbreviation:  -     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-15     Completed Date:  2009-07-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101208439     Medline TA:  Curr Neurovasc Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  95-103     Citation Subset:  IM    
Affiliation:
Department of Physiology, School of Medicine in Katowice, Medical University of Silesia, Poland. agulab@tlen.pl
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Antibodies / therapeutic use
Basilar Artery / pathology
Blood Vessels / drug effects,  pathology*,  physiopathology
Disease Models, Animal
Interleukin-1beta / immunology*
Male
Rats
Rats, Wistar
Subarachnoid Hemorrhage / complications*,  drug therapy
Time Factors
Vasoconstriction / drug effects
Vasospasm, Intracranial / drug therapy,  etiology*,  metabolism*,  pathology*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Interleukin-1beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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