Document Detail


Neutral protease activity in lymphocytes of Lewis rats with acute experimental allergic encephalomyelitis.
MedLine Citation:
PMID:  94652     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lymphocytes from popliteal and inguinal lymph nodes of Lewis rats with acute EAE as a result of injection of lyophilized guinea pig myelin in Freund's complete adjuvant exerted strong proteolytic activity at neutral pH toward myelin basic protein. After injection of myelin the level of proteolytic activity remained about the same as that in lymphocytes from Freund's adjuvant-injected controls until about day 10 after injection, just before the onset of paralytic symptoms; then the proteolytic activity increased to approximately double its former level. Myelin basic protein was hydrolyzed by whole lymphocytes, but more activity was unmasked by homogenization. Similar results were also obtained using lymphocytes from thymus of EAE and control animals. Lymphocytes with high levels of proteolytic activity were not absorbed by glass wool, did not stain with neutral red, nor did they phagocytose antibody-coated sheep red blood cells. Thymus and lymph node lymphocytes cleaved myelin basic protein to three major peptides and a fourth minor peptide, while spleen lymphocytes hydrolyzed basic protein at only one point resulting in two peptides whose molecular weights added up to that of myelin basic protein. The protease activity was inhibited by 5 X 10(-3) M p-chloromercuribenzoate and by phenylmethyl sulfonyl fluoride, TPCK, and soybean trypsin inhibitor, therefore the enzymatic activity probably depends on a serine residue and a sulfhydryl group. The bulk of the enzymatic activity is mostly membrane bound with the highest specific activity and total activity contained in a lysosomal-mitochondrial fraction. In view of the infiltration of lymphocytes into the brain substance in acute EAE, it is suggested that these cells may contribute to the destruction of myelin which is usually attributed to the monocyte or macrophage.
Authors:
M E Smith
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  4     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-07-28     Completed Date:  1980-07-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  689-702     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocytes / enzymology*
Cell Membrane / enzymology
Electrophoresis, Polyacrylamide Gel
Encephalomyelitis, Autoimmune, Experimental / enzymology*
Enzyme Inhibitors
Female
Freund's Adjuvant
Lysosomes / enzymology
Male
Myelin Basic Proteins / metabolism
Peptide Hydrolases / metabolism*
Protease Inhibitors
Rats
Rats, Inbred Lew
Sodium Dodecyl Sulfate
T-Lymphocytes / enzymology
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Myelin Basic Proteins; 0/Protease Inhibitors; 151-21-3/Sodium Dodecyl Sulfate; 9007-81-2/Freund's Adjuvant; EC 3.4.-/Peptide Hydrolases

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