Document Detail

Neutral lipid composition changes in the fat bodies of engorged females Rhipicephalus microplus ticks in response to fungal infections.
MedLine Citation:
PMID:  23138474     Owner:  NLM     Status:  Publisher    
The tick's fat body plays an essential role in energy storage and utilization. This study aimed to analyze the fat body neutral lipid composition in Rhipicephalus microplus engorged females. In the first study (physiological profile of untreated ticks), the lipid analysis took place over the course of 4 days; the engorged females were incubated at optimal conditions and their fat bodies were dissected daily. Fat body lipid analysis after fungal infection with Metarhizium anisopliae sensu lato (s.l.) or Beauveria bassiana s.l. was performed with four groups: one without any treatment, one that was inoculated with a solution of 0.1 % Tween 80 in water, and two groups that were inoculated with M. anisopliae or B. bassiana conidial suspensions. The fat bodies were dissected 24 and 48 h after infection. Lipid analysis was conducted by thin-layer chromatography on a silica plate. The results of the physiological profile showed that the amounts of triacylglycerol (TAG) and free cholesterol (CHO) decreased with time, whereas cholesterol ester (CHOE) increased on the second and fourth days. Following M. anisopliae or B. bassiana infection, there was an increase in the amount of CHO after 24 h, whereas the other lipid classes were not altered. M. anisopliae caused an increase in CHOE and TAG and a reduction in CHO at 48 h after infection; however, B. bassiana infection did not cause significant alterations in the concentrations of these lipids. M. anisopliae and B. bassiana infection changed the fat body metabolism of engorged female R. microplus ticks. This study provides the first report of changes in the neutral lipid composition of the R. microplus fat body.
Isabele C Angelo; Patrícia S Gôlo; Wendell M S Perinotto; Mariana G Camargo; Simone Quinelato; Fillipe A Sá; Emerson G Pontes; Vânia R E P Bittencourt
Related Documents :
12926784 - Fermented and nonfermented liquid feed to growing pigs: effect on aspects of gastrointe...
15890804 - Effects of feeding a blend of grains naturally contaminated with fusarium mycotoxins on...
2566434 - Effect of continuous and intermittent clenbuterol feeding on rat growth rate and muscle.
605074 - Evaluation of aureomycin for prevention of arthritic, pulmonary and cranial forms of fo...
21292834 - Eplerenone suppresses aldosterone/ salt-induced expression of nox-4.
24912134 - Macronutrient selection by seven inbred mouse strains and three taste-related knockout ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-9
Journal Detail:
Title:  Parasitology research     Volume:  -     ISSN:  1432-1955     ISO Abbreviation:  Parasitol. Res.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8703571     Medline TA:  Parasitol Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Animal Parasitology Department, Veterinary Institute, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Comment on "Electronic properties and charge transfer phenomena in Pt nanoparticles on ?-Al(2)O(3): ...
Next Document:  Processing, morphology and water-up of Nafion®/ex-situ Stöber silica nanocomposite membranes as a fu...