| Neutral glycosphingolipid content of ovine milk. | |
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MedLine Citation:
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PMID: 20059899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Milk glycosphingolipids (GSL) have been reported to participate in the newborn's defense against pathogens. Taking this into account, in this study we determined the neutral GSL content of ovine milk, including its fatty acid profile. Its role in bacterial adhesion was also addressed by immunodetection of separate GSL in a high-performance thin-layer chromatography overlay assay. Ovine milk has a neutral GSL pattern similar to human milk and includes lactosylceramide (LacCer; 45.7%), monohexosylceramide (glucosylceramide and galactosylceramide, 31.2%), globotriaosylceramide (Gb3; 19.1%), and globotetraosylceramide (Gb4; 3.5%). Globotriaosylceramide and Gb4 are present in human but not bovine milk. Neutral GSL contained C23:0 and C24:0 as the most abundant fatty acids, a finding consistent with its high content of very long chain fatty acids (longer than C20). Most fatty acids were saturated and had a low content of polyunsaturated fatty acids. Bovine enterotoxigenic Escherichia coli strains bound strongly to LacCer and showed a weak binding to monohexosylceramide. The K99 strain also bound strongly to Gb3, and F41 to Gb4. Lactosylceramide, monohexosylceramide, and Gb3 were also observed to bind to human uropathogenic E. coli strains. The results reported here show the ability of neutral GSL in ovine milk to bind to E. coli strains. These compounds could be used as an alternative and available source to supplement infant or bovine formulas with a view to preventing bacterial infections. |
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Authors:
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L Zancada; F S?nchez-Juanes; J M Alonso; P Hueso |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of dairy science Volume: 93 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-11 Completed Date: 2010-04-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 19-26 Citation Subset: IM |
Copyright Information:
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Copyright 2010 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Departamento de Bioqu?mica y Biolog?a Molecular, Facultad de Biolog?a, Universidad de Salamanca, 37007 Salamanca, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Escherichia coli / metabolism Fatty Acids / analysis Humans Milk / chemistry*, microbiology Neutral Glycosphingolipids / analysis*, chemistry* Sheep |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Neutral Glycosphingolipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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