Document Detail


Neutral fucolipids and fucogangliosides of rat hepatoma HTC and H35 cells, rat liver, and hepatocytes.
MedLine Citation:
PMID:  445466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neutral fucolipids and fucogangliosides of normal rat liver, normal rat hepatocytes, and rat hepatoma H35 and HTC cells have been compared. H35 cells were characterized by accumulation of a relatively large quantity of a few neutral fucolipids and a major fucoganglioside. HTC cells were characterized by accumulation of several neutral fucolipids and by the absence of fucogangliosides. Those fucolipids which accumulated in hepatoma cells were absent in normal rat liver and normal hepatocytes. Normal hepatocytes and normal rat liver contained a low quantity of fucolipids which migrated differently on thin-layer chromatography from those which accumulated in hepatoma cells. In addition to these qualitative differences, the quantity of total fucose in purified glycolipid fraction was much higher in H35 and significantly higher in HTC cells than that in rat liver. A fucoganglioside which accumulated in H35 cells was tentatively characterized as a fucosylated GM1 ganglioside with the following structure: (formula: see text). The results indicate that anomalous fucosylation on glycolipid may take place in hepatoma cells.
Authors:
H Baumann; E Nudelman; K Watanabe; S Hakomori
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  39     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1979-09-01     Completed Date:  1979-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2637-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cells, Cultured
Chromatography, Gel
Fucose / analysis*
Gangliosides / analysis*
Glycolipids / analysis*
Liver / analysis*
Liver Neoplasms, Experimental / analysis*
Rats
Sphingosine / analysis
Chemical
Reg. No./Substance:
0/Gangliosides; 0/Glycolipids; 123-78-4/Sphingosine; 3713-31-3/Fucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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