| Neurovirulence for rats of the JHMV variants escaped from neutralization with the S1-specific monoclonal antibodies. | |
| | |
MedLine Citation:
|
PMID: 8830478 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We have studied the neurovirulence for rats of the MAb-resistant variants isolated from a highly neurovirulent JHMV, cl-2. The variants, MM6 and MM13, with point mutation located within the N terminal 100 amino acids (aa) of the S1 protein showed no alteration in neurovirulence in comparison with cl-2, showing high neurovirulence. The variants, MM65 and MM85, with a deletion composed of about 150 aa located in the middle of the S1 subunit were revealed to be non-neurovirulent. A variant MM78 with one aa deletion, asparagic acid at number 543 from the N terminus of the S1, was shown to be low-virulence. The neurovirulence of these viruses paralleled with the viral growth potential in the rat brain. However, all of these variants as well as parental cl-2 showed high neurovirulence for mice. These results suggest that the domain composed of about 150 aa in the middle of the S1 is critical for high-neurovirulence of JHMV for rats. |
| | |
Authors:
|
F Taguchi; H Suzuki; H Takahashi; H Kubo |
Related Documents
:
|
7037168 - Colchicine affects the distribution of isotope-labeled h2o and extracellular markers in... 2386938 - 2-acetylaminofluorene-mediated alteration in the level of liver arylsulfotransferase iv... 176398 - Stimulation of growth of a transplantable hepatoma in rats by parenteral nutrition. 1624078 - Endotoxin levels in cirrhotic rats with sterile and infected ascites. 2863328 - Elevation of rna coding for tyrosine hydroxylase in rat adrenal gland by reserpine trea... 8557058 - Cellular mechanisms underlying the increased duodenal iron absorption in rats in respon... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Advances in experimental medicine and biology Volume: 380 ISSN: 0065-2598 ISO Abbreviation: Adv. Exp. Med. Biol. Publication Date: 1995 |
Date Detail:
|
Created Date: 1996-10-16 Completed Date: 1996-10-16 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 0121103 Medline TA: Adv Exp Med Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 185-7 Citation Subset: IM |
Affiliation:
|
National Institute of Neuroscience, NCNP, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibodies, Monoclonal* Antibody Specificity Coronavirus Infections / immunology, physiopathology* Genetic Variation* Membrane Glycoproteins / genetics*, immunology Mice Murine hepatitis virus / genetics, immunology*, pathogenicity* Neutralization Tests Point Mutation Rats Sequence Deletion Species Specificity Viral Envelope Proteins / genetics*, immunology Virulence |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Membrane Glycoproteins; 0/Viral Envelope Proteins; 107476-75-5/spike glycoprotein, coronavirus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transcription and translation of proinflammatory cytokines following JHMV infection.
Next Document: T cell immunodeficiency involved in pathogenicity of attenuated MHV3 mutants.