Document Detail


Neurotoxicity of 25-OH-cholesterol on NGF-differentiated PC12 cells.
MedLine Citation:
PMID:  9482261     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PC12 cells induced to differentiate with nerve growth factor were used to study the neurotoxicity of 25-OH-cholesterol. This agent induced a dose- and time-dependent cell death in neuronal PC12 cells. Cells treated with this agent showed condensed nuclei, a morphology similar to that of cells dying of programmed cell death. However, agents known to prevent neuronal programmed cell death (cyclic AMP, KCl, aurintricarboxylic acid, and cycloheximide) failed to prevent the 25-OH-cholesterol-mediated cytotoxicity. On the other hand, cell death induced by 25-OH-cholesterol was prevented by treatment with vitamin E and methyl-beta-cyclodextrin. In contrast to observations made in other cell types, whole-cell patch clamp recording of neuronal PC12 cells revealed that treatment with 25-OH-cholesterol did not significantly alter calcium influx through voltage-dependent channels. These results provide the first characterization of the toxicity of cholesterol oxides toward neuronal PC12 cells, which should be useful in future studies on the interactions between cholesterol oxides and cells from the nervous system.
Authors:
J Y Chang; K D Phelan; L Z Liu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  23     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-04-03     Completed Date:  1998-04-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7-16     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Ascorbic Acid / pharmacology
Aurintricarboxylic Acid / pharmacology
Blood
Calcium Channels / drug effects
Cell Differentiation*
Cyclic AMP / pharmacology
Cyclodextrins / pharmacology
Hydroxycholesterols / pharmacology*
Nerve Growth Factors / pharmacology*
PC12 Cells / cytology,  drug effects*
Potassium Chloride / pharmacology
Rats
Vitamin E / pharmacology
beta-Cyclodextrins*
Grant Support
ID/Acronym/Agency:
NS32253/NS/NINDS NIH HHS; NS33959/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Calcium Channels; 0/Cyclodextrins; 0/Hydroxycholesterols; 0/Nerve Growth Factors; 0/beta-Cyclodextrins; 0/methyl-beta-cyclodextrin; 1406-18-4/Vitamin E; 2140-46-7/25-hydroxycholesterol; 4431-00-9/Aurintricarboxylic Acid; 50-81-7/Ascorbic Acid; 60-92-4/Cyclic AMP; 7447-40-7/Potassium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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