Document Detail


Neurotensin selectively facilitates glutamatergic transmission in globus pallidus.
MedLine Citation:
PMID:  16814931     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The tridecapeptide neurotensin has been demonstrated to modulate neurotransmission in a number of brain regions. There is evidence that neurotensin receptors exist in globus pallidus presynaptically and postsynaptically. Whole-cell patch-clamp recordings were used to investigate the modulatory effects of neurotensin on glutamate and GABA transmission in this basal ganglia nucleus in rats. Neurotensin at 1 microM significantly increased the frequency of glutamate receptor-mediated miniature excitatory postsynaptic currents. In contrast, neurotensin had no effect on GABA(A) receptor-mediated miniature inhibitory postsynaptic currents. The presynaptic facilitation of neurotensin on glutamatergic transmission could be mimicked by the C-terminal fragment, neurotensin (8-13), but not by the N-terminal fragment, neurotensin (1-8). The selective neurotensin type-1 receptor antagonist, SR48692 {2-[(1-(7-chloro-4-quinolinyl)-5-2(2,6-dimethoxyphenyl)pyrazol-3-yl)carbonylamino]-tricyclo(3.3.1.1.(3.7))-decan-2-carboxylic acid}, blocked this facilitatory effect of neurotensin, and which itself had no effect on miniature excitatory postsynaptic currents. The specific phospholipase C inhibitor, U73122 {1-[6-[[17beta-3-methoyyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1H-pyrrole-2,5-dione}, significantly inhibit neurotensin-induced facilitation on glutamate release. Taken together with the reported postsynaptic depolarization of neurotensin in globus pallidus, it is suggested that neurotensin excites the globus pallidus neurons by multiple mechanisms which may provide a rationale for further investigations into its involvement in motor disorders originating from the basal ganglia.
Authors:
L Chen; K K L Yung; W H Yung
Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-11
Journal Detail:
Title:  Neuroscience     Volume:  141     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-21     Completed Date:  2006-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1871-8     Citation Subset:  IM    
Affiliation:
Department of Physiology, The Chinese University of Hong Kong, Shatin, Hong Kong, China. chenleiqd@163.com
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MeSH Terms
Descriptor/Qualifier:
Anesthetics, Local / pharmacology
Animals
Animals, Newborn
Bicuculline / pharmacology
Dose-Response Relationship, Radiation
Drug Interactions
Electric Stimulation / methods
Enzyme Inhibitors / pharmacology
Estrenes / pharmacology
Excitatory Postsynaptic Potentials / drug effects,  physiology,  radiation effects
GABA Antagonists / pharmacology
Globus Pallidus / cytology*
Glutamic Acid / metabolism*
Neural Inhibition / drug effects,  physiology,  radiation effects
Neurons / drug effects*,  metabolism
Neurotensin / antagonists & inhibitors,  pharmacology*
Patch-Clamp Techniques / methods
Pyrazoles / pharmacology
Pyrrolidinones / pharmacology
Quinolines / pharmacology
Rats
Rats, Sprague-Dawley
Synaptic Transmission / drug effects*
Tetrodotoxin / pharmacology
Chemical
Reg. No./Substance:
0/Anesthetics, Local; 0/Enzyme Inhibitors; 0/Estrenes; 0/GABA Antagonists; 0/Pyrazoles; 0/Pyrrolidinones; 0/Quinolines; 112648-68-7/1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; 146362-70-1/SR 48692; 39379-15-2/Neurotensin; 4368-28-9/Tetrodotoxin; 485-49-4/Bicuculline; 56-86-0/Glutamic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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