Document Detail


Neurosteroids do not regulate proopiomelanocortin-gene expression in pituitary cells.
MedLine Citation:
PMID:  8223985     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Brain-derived steroids (neurosteroids) constitute a new group of steroids synthesized in brain cells independently from peripheral sources. While the membraneous actions of these steroids have been characterized in some detail, their genomic effects are less well studied up to now. In the present study, the action of the neurosteroids dehydroepiandrosterone, pregnenolone and pregnanolone on proopiomelanocortin (POMC)-gene expression was investigated in pituitary AtT 20 cells, which were cultured under serum-free conditions and at strictly controlled steroid levels. In these cells, POMC-gene expression is known to be negatively regulated by adrenocortical glucocorticoids. Quantitative Northern blot analysis showed that none of these neurosteroids or their conjugates affected POMC-mRNA levels even at the highest concentrations employed. The 21-OH steroid corticosterone was used as a control and markedly suppressed the synthesis of POMC-mRNA. These results suggest that neurosteroids do not control POMC-gene expression at the level of the pituitary corticotroph and exclude significant effects of these compounds at the glucocorticoid receptor.
Authors:
H Vedder; F Parlow; W Zieglgänsberger; F Holsboer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental and clinical endocrinology     Volume:  101     ISSN:  0232-7384     ISO Abbreviation:  Exp. Clin. Endocrinol.     Publication Date:  1993  
Date Detail:
Created Date:  1993-12-20     Completed Date:  1993-12-20     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8302802     Medline TA:  Exp Clin Endocrinol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  161-5     Citation Subset:  IM    
Affiliation:
Department of Neuroendocrinology, Max Planck Institute of Psychiatry, Munich, FRG.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Northern
Brain Chemistry*
Cells, Cultured
Corticosterone / pharmacology
Dehydroepiandrosterone / pharmacology
Gene Expression / drug effects
Mice
Pituitary Gland / cytology,  drug effects,  metabolism*
Pregnanolone / pharmacology
Pregnenolone / pharmacology
Pro-Opiomelanocortin / biosynthesis*,  genetics
RNA, Messenger / biosynthesis
Steroids / pharmacology*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Steroids; 128-20-1/Pregnanolone; 145-13-1/Pregnenolone; 50-22-6/Corticosterone; 53-43-0/Dehydroepiandrosterone; 66796-54-1/Pro-Opiomelanocortin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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