Document Detail

Neurosphere cell differentiation to aldynoglia promoted by olfactory ensheathing cell conditioned medium.
MedLine Citation:
PMID:  19235256     Owner:  NLM     Status:  MEDLINE    
Cells from central nervous system with morphology similar to radial glia and properties intermediate between astrocytes and Schwann cells were called growth-promoting glia or aldynoglia. These cells are present in adult brain olfactory bulb, hypothalamus, hypophysis, pineal gland and in the developing brain, and spinal cord (Cameron and Rakic (1991) Glia 4:124-137; Gudiño-Cabrera and Nieto-Sampedro (2000) 30:49-63). We report now that neurosphere cells, abundantly generated from E15 rat or E13 mouse corpus striatum, differentiate to aldynoglia-like cells when plated onto an adhesive substrate, and cultured in the presence of olfactory ensheathing cell conditioned medium, supplemented with EGF and bFGF. The immunophenotype, mRNA expression (microarray and RT-PCR analysis), neurite growth-promoting and ensheathing properties of the cells derived from neurospheres were similar to those of aldynoglia. Neurosphere-derived, aldynoglia-like cells expressed, with respect to neurospheres, very increased levels of GFAP, high levels of nestin and vimentin, extracellular matrix proteins, and inhibitors of the catabolism of those extracellular matrix proteins.
Ernesto Doncel-Pérez; Sara Caballero-Chacón; Manuel Nieto-Sampedro
Related Documents :
19235256 - Neurosphere cell differentiation to aldynoglia promoted by olfactory ensheathing cell c...
20674606 - Reconstruction and quantitative evaluation of dopaminergic innervation of striatal neur...
19388346 - Thrombin enhances glioma growth.
19535576 - Beta4 tubulin identifies a primitive cell source for oligodendrocytes in the mammalian ...
16619236 - Hydralazine rescues pc12 cells from acrolein-mediated death.
3100506 - Cell-density-dependent killing of myxococcus xanthus by autocide amv.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glia     Volume:  57     ISSN:  1098-1136     ISO Abbreviation:  Glia     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-10-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8806785     Medline TA:  Glia     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1393-409     Citation Subset:  IM    
Copyright Information:
(c) 2009 Wiley-Liss, Inc.
Neural Plasticity Group, Cajal Institute, CSIC, Madrid, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Adhesion / genetics,  physiology
Cell Differentiation / physiology*
Cell Movement
Cells, Cultured
Coculture Techniques
Corpus Striatum / cytology*,  physiology
Culture Media, Conditioned
Ganglia, Spinal / physiology
Green Fluorescent Proteins / genetics
Mice, Inbred C57BL
Mice, Transgenic
Myelin Sheath / physiology
Neurites / physiology
Neuroglia / cytology,  physiology*
Neurons / physiology
Olfactory Bulb / cytology*,  physiology
Rats, Wistar
Reg. No./Substance:
0/Culture Media, Conditioned; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Low-dose azacitidine after allogeneic stem cell transplantation for acute leukemia.
Next Document:  Evaluation of the ERETIC method as an improved quantitative reference for 1H HR-MAS spectroscopy of ...