| Neuroprotective properties of marrow-isolated adult multilineage-inducible cells in rat hippocampus following global cerebral ischemia are enhanced when complexed to biomimetic microcarriers. | |
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MedLine Citation:
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PMID: 21496021 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell-based therapies for global cerebral ischemia represent promising approaches for neuronal damage prevention and tissue repair promotion. We examined the potential of marrow-isolated adult multilineage-inducible (MIAMI) cells, a homogeneous subpopulation of immature human mesenchymal stromal cell, injected into the hippocampus to prevent neuronal damage induced by global ischemia using rat organotypic hippocampal slices exposed to oxygen-glucose deprivation and rats subjected to asphyxial cardiac arrest. We next examined the value of combining fibronectin-coated biomimetic microcarriers (FN-BMMs) with epidermal growth factor (EGF)/basic fibroblast growth factor (bFGF) pre-treated MIAMI compared to EGF/bFGF pre-treated MIAMI cells alone, for their in vitro and in vivo neuroprotective capacity. Naïve and EGF/bFGF pre-treated MIAMI cells significantly protected the Cornu Ammonis layer 1 (CA1) against ischemic death in hippocampal slices and increased CA1 survival in rats. MIAMI cells therapeutic value was significantly increased when delivering the cells complexed with FN-BMMs, probably by increasing stem cell survival and paracrine secretion of pro-survival and/or anti-inflammatory molecules as concluded from survival, differentiation and gene expression analysis. Four days after oxygen and glucose deprivation and asphyxial cardiac arrest, few transplanted cells administered alone survived in the brain whereas stem cell survival improved when injected complexed with FN-BMMs. Interestingly, a large fraction of the transplanted cells administered alone or in complexes expressed βIII-tubulin suggesting that partial neuronal transdifferentiation may be a contributing factor to the neuroprotective mechanism of MIAMI cells. |
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Authors:
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Elisa Garbayo; Ami P Raval; Kevin M Curtis; David Della-Morte; Lourdes A Gomez; Gianluca D'Ippolito; Teresita Reiner; Carlos Perez-Stable; Guy A Howard; Miguel A Perez-Pinzon; Claudia N Montero-Menei; Paul C Schiller |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-05-13 |
Journal Detail:
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Title: Journal of neurochemistry Volume: 119 ISSN: 1471-4159 ISO Abbreviation: J. Neurochem. Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-11-15 Completed Date: 2012-01-09 Revised Date: 2012-04-13 |
Medline Journal Info:
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Nlm Unique ID: 2985190R Medline TA: J Neurochem Country: England |
Other Details:
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Languages: eng Pagination: 972-88 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry. |
Affiliation:
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Department of Medicine, University of Miami Miller School of Medicine, Miami, Florida, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomimetic Materials / pharmacology* Bone Marrow Cells / cytology Brain Ischemia / pathology*, therapy* Cell Differentiation / physiology* Cells, Cultured Hippocampus / cytology* Humans Lactic Acid / pharmacology Male Mesenchymal Stem Cell Transplantation / methods* Mesenchymal Stem Cells / cytology Multipotent Stem Cells / cytology* Neurons / pathology Organ Culture Techniques Polyglycolic Acid / pharmacology Rats Transplantation, Heterologous / methods Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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NS054147/NS/NINDS NIH HHS; NS34773/NS/NINDS NIH HHS; NS45676/NS/NINDS NIH HHS; R01 NS034773-13/NS/NINDS NIH HHS; R01 NS045676-07/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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