Document Detail


Neuroprotective properties of marrow-isolated adult multilineage-inducible cells in rat hippocampus following global cerebral ischemia are enhanced when complexed to biomimetic microcarriers.
MedLine Citation:
PMID:  21496021     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-based therapies for global cerebral ischemia represent promising approaches for neuronal damage prevention and tissue repair promotion. We examined the potential of marrow-isolated adult multilineage-inducible (MIAMI) cells, a homogeneous subpopulation of immature human mesenchymal stromal cell, injected into the hippocampus to prevent neuronal damage induced by global ischemia using rat organotypic hippocampal slices exposed to oxygen-glucose deprivation and rats subjected to asphyxial cardiac arrest. We next examined the value of combining fibronectin-coated biomimetic microcarriers (FN-BMMs) with epidermal growth factor (EGF)/basic fibroblast growth factor (bFGF) pre-treated MIAMI compared to EGF/bFGF pre-treated MIAMI cells alone, for their in vitro and in vivo neuroprotective capacity. Naïve and EGF/bFGF pre-treated MIAMI cells significantly protected the Cornu Ammonis layer 1 (CA1) against ischemic death in hippocampal slices and increased CA1 survival in rats. MIAMI cells therapeutic value was significantly increased when delivering the cells complexed with FN-BMMs, probably by increasing stem cell survival and paracrine secretion of pro-survival and/or anti-inflammatory molecules as concluded from survival, differentiation and gene expression analysis. Four days after oxygen and glucose deprivation and asphyxial cardiac arrest, few transplanted cells administered alone survived in the brain whereas stem cell survival improved when injected complexed with FN-BMMs. Interestingly, a large fraction of the transplanted cells administered alone or in complexes expressed βIII-tubulin suggesting that partial neuronal transdifferentiation may be a contributing factor to the neuroprotective mechanism of MIAMI cells.
Authors:
Elisa Garbayo; Ami P Raval; Kevin M Curtis; David Della-Morte; Lourdes A Gomez; Gianluca D'Ippolito; Teresita Reiner; Carlos Perez-Stable; Guy A Howard; Miguel A Perez-Pinzon; Claudia N Montero-Menei; Paul C Schiller
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-05-13
Journal Detail:
Title:  Journal of neurochemistry     Volume:  119     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-15     Completed Date:  2012-01-09     Revised Date:  2014-05-21    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  972-88     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomimetic Materials / pharmacology*
Bone Marrow Cells / cytology
Brain Ischemia / pathology*,  therapy*
Cell Differentiation / physiology*
Cells, Cultured
Hippocampus / cytology*
Humans
Lactic Acid / pharmacology
Male
Mesenchymal Stem Cell Transplantation / methods*
Mesenchymal Stromal Cells / cytology
Multipotent Stem Cells / cytology*
Neurons / pathology
Organ Culture Techniques
Polyglycolic Acid / pharmacology
Rats
Transplantation, Heterologous / methods
Young Adult
Grant Support
ID/Acronym/Agency:
NS054147/NS/NINDS NIH HHS; NS34773/NS/NINDS NIH HHS; NS45676/NS/NINDS NIH HHS; R01 NS034773/NS/NINDS NIH HHS; R01 NS034773-13/NS/NINDS NIH HHS; R01 NS045676/NS/NINDS NIH HHS; R01 NS045676-07/NS/NINDS NIH HHS; R01 NS054147/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/polylactic acid-polyglycolic acid copolymer; 26009-03-0/Polyglycolic Acid; 33X04XA5AT/Lactic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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