Document Detail


Neuroprotective effects of the citrus flavanones against H2O2-induced cytotoxicity in PC12 cells.
MedLine Citation:
PMID:  18189359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The citrus flavanones hesperidin, hesperetin, and neohesperidin are known to exhibit antioxidant activities and could traverse the blood-brain barrier. H2O2 formation induces cellular oxidative stress associated with neurodegenerative diseases. In this study, protective effects of pretreatments (6 h) with hesperidin, hesperetin, and neohesperidin (0.8, 4, 20, and 50 microM) on H2O2-induced (400 microM, 16 h) neurotoxicity in PC12 cells were evaluated. The results showed that hesperetin, hesperidin, and neohesperidin, at all test concentrations, significantly ( p < 0.05) inhibited the decrease of cell viability (MTT reduction), prevented membrane damage (LDH release), scavenged ROS formation, increased catalase activity, and attenuated the elevation of intracellular free Ca2+, the decrease of mitochondrial membrane potential (except those of 0.8 microM neohesperidin-treated cells) and the increase of caspase-3 activity in H2O2-induced PC12 cells. Meanwhile, hesperidin and hesperetin attenuated decreases of glutathione peroxidase and glutathione reductase activities and decreased DNA damage in H2O2-induced PC12 cells. These results first demonstrate that the citrus flavanones hesperidin, hesperetin, and neohesperidin, even at physiological concentrations, have neuroprotective effects against H2O2-induced cytotoxicity in PC12 cells. These dietary antioxidants are potential candidates for use in the intervention for neurodegenerative diseases.
Authors:
Sam-Long Hwang; Gow-Chin Yen
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Publication Detail:
Type:  Journal Article     Date:  2008-01-12
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  56     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-06     Completed Date:  2008-04-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  859-64     Citation Subset:  IM    
Affiliation:
Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antioxidants / metabolism
Calcium / metabolism
Caspase 3 / metabolism
Cell Death / drug effects*
Citrus / chemistry*
DNA Damage / drug effects
Flavanones / pharmacology*
Hydrogen Peroxide / pharmacology*
Matrix Metalloproteinases / metabolism
Neuroprotective Agents / pharmacology*
PC12 Cells
Rats
Reactive Oxygen Species / metabolism
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Flavanones; 0/Neuroprotective Agents; 0/Reactive Oxygen Species; 7440-70-2/Calcium; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/Caspase 3; EC 3.4.24.-/Matrix Metalloproteinases

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