| Neuroprotective effects of chronic exposure of SH-SY5Y to low lithium concentration involve glycolysis stimulation, extracellular pyruvate accumulation and resistance to oxidative stress. | |
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MedLine Citation:
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PMID: 22436355 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Recent studies suggest that lithium protects neurons from death induced by a wide array of neurotoxic insults, stimulates neurogenesis and could be used to prevent age-related neurodegenerative diseases. In this study, SH-SY5Y human neuronal cells were cultured in the absence (Con) or in the presence (Li+) of a low lithium concentration (0.5 mm Li2CO3, i.e. 1 mm lithium ion) for 25-50 wk. In the course of treatment, growth rate of Con and Li+ cells was regularly analysed using Alamar Blue dye. Resistance to oxidative stress was investigated by evaluating: (1) the adverse effects of high concentrations of lithium (4-8 mm) or glutamate (20-90 mm) on cell growth rate; (2) the levels of lipid peroxidation (TBARS) and total glutathione; (3) the expression levels of the anti-apoptotic Bcl-2 protein. In addition, glucose metabolism was investigated by analysing selected metabolites in culture media and cell extracts by 1H-NMR spectroscopy. As compared to Con, Li+ cells multiplied faster and were more resistant to stress, as evidenced by a lower dose-dependent decrease of Alamar Blue reduction and dose-dependent increase of TBARS levels induced by toxic doses of lithium and glutamate. Total glutathione content and Bcl-2 level were increased in Li+ cells. Glucose consumption and glycolytic activity were enhanced in Li+ cells and an important release of pyruvate was observed. We conclude that chronic exposure to lithium induces adaptive changes in metabolism of SH-SY5Y cells involving a higher cell growth rate and a better resistance to oxidative stress. |
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Authors:
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Riadh Nciri; Frank Desmoulin; Mohamed Saleh Allagui; Jean-Claude Murat; Abdelfattah El Feki; Christian Vincent; Françoise Croute |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-3-22 |
Journal Detail:
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Title: The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP) Volume: - ISSN: 1469-5111 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-3-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815893 Medline TA: Int J Neuropsychopharmacol Country: - |
Other Details:
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Languages: ENG Pagination: 1-12 Citation Subset: - |
Affiliation:
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Laboratoire de Biologie Cellulaire, Faculté de Médecine Purpan, Université Paul Sabatier Toulouse III, 37 Allées Jules Guesde, Toulouse, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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