| Neuroprotective effect of cilostazol against focal cerebral ischemia via antiapoptotic action in rats. | |
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MedLine Citation:
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PMID: 11861782 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examined the protective effects of cilostazol on cerebral infarcts produced by subjecting rats to 2-h occlusion of the left middle cerebral artery followed by 24-h reperfusion. The ischemic cerebral infarct consistently involved the cortex and striatum. The infarct size was significantly reduced, when rats received 10 mg/kg cilostazol intravenously 5 min or 1 h after the completion of 2-h ischemia. Cyclic AMP level was significantly elevated in the cortex of 4- and 12-h reperfusion (P < 0.01) following treatment with cilostazol (10 mg/kg, 5 min after 2-h ischemia) accompanied by decreased tumor necrosis factor-alpha level. Samples from the regions corresponding to the penumbra showed markedly reduced Bcl-2 protein level and, in contrast, high levels of Bax protein and cytochrome c release. Cilostazol decreased Bax protein and cytochrome c release and increased the levels of Bcl-2 protein. Cilostazol (10(-7)-10(-5) M) potently and concentration dependently scavenged hydroxyl and peroxyl radicals. In conclusion, cilostazol treatment decreases ischemic brain infarction in association with inhibition of apoptotic and oxidative cell death. |
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Authors:
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Jae Moon Choi; Hwa Kyoung Shin; Ki Young Kim; Jeong Hyun Lee; Ki Whan Hong |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of pharmacology and experimental therapeutics Volume: 300 ISSN: 0022-3565 ISO Abbreviation: J. Pharmacol. Exp. Ther. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-02-25 Completed Date: 2002-03-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376362 Medline TA: J Pharmacol Exp Ther Country: United States |
Other Details:
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Languages: eng Pagination: 787-93 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, College of Medicine, and Research Institute of Genetic Engineering, Pusan National University, Pusan, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects* Blotting, Western Brain Infarction / pathology, prevention & control Cyclic AMP / metabolism Cytochrome c Group / metabolism DNA Fragmentation / drug effects Free Radical Scavengers / metabolism Genes, bcl-2 Ischemic Attack, Transient / drug therapy*, pathology* Male Mitochondria / metabolism Neuroprotective Agents / therapeutic use* Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-bcl-2* Rats Rats, Sprague-Dawley Reperfusion Injury / pathology, prevention & control Tetrazoles / therapeutic use* Tumor Necrosis Factor-alpha / metabolism bcl-2-Associated X Protein |
| Chemical | |
Reg. No./Substance:
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0/Bax protein, rat; 0/Cytochrome c Group; 0/Free Radical Scavengers; 0/Neuroprotective Agents; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/Tetrazoles; 0/Tumor Necrosis Factor-alpha; 0/bcl-2-Associated X Protein; 60-92-4/Cyclic AMP; 73963-72-1/cilostazol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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