Document Detail


Neuroprotective effect of antioxidants on ischaemia and reperfusion-induced cerebral injury.
MedLine Citation:
PMID:  12798674     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study is designed to investigate the effect of dietary flavanoid rutin, micronutrient selenium and garlic oil on ischaemia and reperfusion-induced cerebral injury. Global cerebral ischaemia was induced by occluding right and left common carotid arteries for 10min followed by reperfusion for 24h. Cerebral infarct size was estimated using triphenyltetrazolium chloride staining. Elevated plus maze was employed to estimate short-term memory. Degree of motor incoordination was evaluated using inclined beam-walking test and lateral push test. Mitochondrial thiobarbituric acid reactive substances (TBARS) assay was employed as an index of oxidative stress. Global cerebral ischaemia followed by reperfusion produced a significant impairment in short-term memory and motor coordination and produced a notable increase in mitochondrial TBARS. Administration of rutin and garlic oil before global cerebral ischaemia markedly reduced cerebral infarct size and attenuated impairment in short-term memory and motor coordination. Administration of sodium selenite either before or after global cerebral ischaemia markedly reduced cerebral infarct size and attenuated impairment in short-term memory and motor coordination. The protective effect of rutin, sodium selenite and garlic oil was accompanied by a marked decrease in mitochondrial TBARS. On the basis of these results, it may be suggested that rutin and garlic oil administrated before cerebral ischaemia may scavenge reactive oxygen species and consequently attenuate global cerebral ischaemia and reperfusion-induced cerebral injury. Sodium selenite administrated before and after cerebral ischaemia may be neuroprotective due to its antioxidant effect.
Authors:
Ram Gupta; Manjeet Singh; Ajay Sharma
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Pharmacological research : the official journal of the Italian Pharmacological Society     Volume:  48     ISSN:  1043-6618     ISO Abbreviation:  Pharmacol. Res.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-06-11     Completed Date:  2004-06-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907422     Medline TA:  Pharmacol Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  209-15     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, 147002, Punjab, Patiala, India.
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MeSH Terms
Descriptor/Qualifier:
Allyl Compounds / pharmacology,  therapeutic use*
Animals
Antioxidants / pharmacology,  therapeutic use*
Brain Ischemia / drug therapy*
Cerebral Infarction / etiology,  prevention & control*
Data Interpretation, Statistical
Disease Models, Animal
Female
Male
Memory Disorders / etiology,  prevention & control
Memory, Short-Term / drug effects
Mice
Micronutrients / pharmacology,  therapeutic use
Mitochondria / metabolism
Reactive Oxygen Species / metabolism
Reperfusion Injury / complications,  drug therapy*
Rutin / pharmacology,  therapeutic use*
Sodium Selenite / pharmacology,  therapeutic use*
Sulfides / pharmacology,  therapeutic use*
Thiobarbituric Acid Reactive Substances / metabolism
Chemical
Reg. No./Substance:
0/Allyl Compounds; 0/Antioxidants; 0/Micronutrients; 0/Reactive Oxygen Species; 0/Sulfides; 0/Thiobarbituric Acid Reactive Substances; 10102-18-8/Sodium Selenite; 153-18-4/Rutin; 592-88-1/allyl sulfide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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