Document Detail


Neuroprotective effect of alpha-lipoic acid on hydrostatic pressure-induced damage of retinal ganglion cells in vitro.
MedLine Citation:
PMID:  22917607     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Recently, alpha-lipoic acid (ALA) has been reported to afford protection against neurodegenerative disorders in humans and experimental animals, yet little study elucidates whether it works in glaucomatous optic neuropathy. OBJECTIVE: This study aims to investigate whether ALA possesses neuroprotection against hydrostatic pressure-induced damage and explore its possible protective mechanism in cultured retinal ganglion cells (RGCs) in vitro. METHODS: RGC-5 cells were differentiated using staurosporine and pre-treated with different concentrations of ALA, then subjected to 50mm Hg hydrostatic pressure for 6h. After elevated hydrostatic pressure, cell viability was measured using MTT assay and apoptosis was evaluated using flow cytometry with Annexin V/PI staining. Intracellular reactive oxygen species (ROS) changes were determined by flow cytometry based on 2',7'-dichlorofluorescein diacetate (DCFH-DA). The expression of manganese superoxide dismutase (MnSOD) was measured via quantitative real-time PCR and Western blotting analysis. RESULTS: Increases of apoptotic rate and ROS production were observed in pressure-treated RGC-5 cells compared to normal control cells. In contrast, pretreatment of ALA significantly reduced the production of ROS, increased the expression of MnSOD and prevented apoptosis in pressure-treated RGC-5 cells. CONCLUSIONS: These findings suggest that there are protective effects of ALA against elevated hydrostatic pressure-induced damage in RGC-5 cells, indicating ALA might be a potential therapeutic agent for glaucomatous optic neuropathy.
Authors:
Bing Liu; Xiaohua Ma; Dadong Guo; Yuanyuan Guo; Ninghong Chen; Hongsheng Bi
Related Documents :
12360157 - Association of angiotensin-converting enzyme dd genotype with blood pressure sensitivit...
17620957 - Job strain, decision latitude and alpha2b-adrenergic receptor polymorphism significantl...
12911327 - Angiotensinogen gene m235t polymorphism and reduction in wall thickness in response to ...
19196567 - Relation between angiotensin-converting enzyme i/d gene polymorphism and pulse pressure...
17444927 - Evaluation of mean arterial blood pressure, heart rate, and sympathetic nerve activity ...
6713917 - Increased intracranial pressure: an indication to decompress a tension pneumomediastinum.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-17
Journal Detail:
Title:  Neuroscience letters     Volume:  -     ISSN:  1872-7972     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ireland Ltd.
Affiliation:
Shandong University of Traditional Chinese Medicine, 16369 Jinan Jingshi Road, Jinan 250014, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Dexmedetomidine decreases hyperalgesia in neuropathic pain by increasing acetylcholine in the spinal...
Next Document:  Effects of valproic acid on the expression of trophic factors in human bone marrow mesenchymal strom...