Document Detail

Neuroprotective effect of adenine on purkinje cell survival in rat cerebellar primary cultures.
MedLine Citation:
PMID:  14635226     Owner:  NLM     Status:  MEDLINE    
Although adenosine or ATP is known to control various physiological functions in the brain, including synaptic transmission, neuronal cell death, and neurite outgrowth via P1 or P2 purinergic receptors in the nervous system, little is known about the functions of many other purine derivatives. We examined the effects of various purines on survival in the cerebellar cortex of Purkinje cells with large cell bodies and highly branched dendrites, and it was found that some purine and pyrimidine derivatives influence Purkinje cell survival. Treatment with adenine, guanine, guanosine, guanine nucleotides, and uracil nucleotides protected Purkinje cells from cell death in the cerebellar primary cultures. Among the effective compounds, adenine had the most potent survival activities on Purkinje cells. Other adenine-based purines such as adenosine, AMP, ADP, and ATP did not promote Purkinje cell survival. Furthermore, metabolic inhibitors of adenine had no effect on the protective ability of adenine for Purkinje cells, suggesting that adenine itself, not adenine metabolites, maintains Purkinje cell survival. These results suggest that adenine is involved in the control of Purkinje cell survival in cerebellar primary cultures via a novel adenine-dependent mechanism.
Shun Watanabe; Yoji Yoshimi; Masahiko Ikekita
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  74     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-11-24     Completed Date:  2004-01-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  754-9     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Department of Applied Biology, Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan.
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MeSH Terms
Adenine / analogs & derivatives*,  pharmacology*
Cell Survival / drug effects
Cells, Cultured
Neuroprotective Agents / pharmacology*
Purines / pharmacology
Purkinje Cells / drug effects*
Pyrimidines / pharmacology
Rats, Wistar
Reg. No./Substance:
0/Neuroprotective Agents; 0/Purines; 0/Pyrimidines; 2715-68-6/9-ethyladenine; 73-24-5/Adenine

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