| Neuropilin-1 interacts with the second branchial arch microenvironment to mediate chick neural crest cell dynamics. | |
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MedLine Citation:
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PMID: 20503363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cranial neural crest cells (NCCs) require neuropilin signaling to reach and invade the branchial arches. Here, we use an in vivo chick model to investigate whether the neuropilin-1 knockdown phenotype is specific to the second branchial arch (ba2), changes in NCC behaviors and phenotypic consequences, and whether neuropilins work together to facilitate entry into and invasion of ba2. We find that cranial NCCs with reduced neuropilin-1 expression displayed shorter protrusions and decreased cell body and nuclear length-to-width ratios characteristic of a loss in polarity and motility, after specific interaction with ba2. Directed NCC migration was rescued by transplantation of transfected NCCs into rhombomere 4 of younger hosts. Lastly, reduction of neuropilin-2 expression by shRNA either solely or with reduction of neuropilin-1 expression did not lead to a stronger head phenotype. Thus, NCCs, independent of rhombomere origin, require neuropilin-1, but not neuropilin-2 to maintain polarity and directed migration into ba2. |
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Authors:
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Rebecca McLennan; Paul M Kulesa |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental dynamics : an official publication of the American Association of Anatomists Volume: 239 ISSN: 1097-0177 ISO Abbreviation: Dev. Dyn. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-26 Completed Date: 2010-09-30 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 9201927 Medline TA: Dev Dyn Country: United States |
Other Details:
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Languages: eng Pagination: 1664-73 Citation Subset: IM |
Affiliation:
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Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Branchial Region / metabolism* Cell Movement Chick Embryo Embryo, Nonmammalian Head Neurons / metabolism* Neuropilin-1 / metabolism* Neuropilin-2 Neuropilins / metabolism Phenotype Signal Transduction Skull / metabolism Syndrome |
| Grant Support | |
ID/Acronym/Agency:
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1R01HD057922/HD/NICHD NIH HHS; R01 HD057922-01A1/HD/NICHD NIH HHS; R01 HD057922-04/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Neuropilin-2; 0/Neuropilins; 144713-63-3/Neuropilin-1 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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