Document Detail


Neuropilin-1 interacts with the second branchial arch microenvironment to mediate chick neural crest cell dynamics.
MedLine Citation:
PMID:  20503363     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cranial neural crest cells (NCCs) require neuropilin signaling to reach and invade the branchial arches. Here, we use an in vivo chick model to investigate whether the neuropilin-1 knockdown phenotype is specific to the second branchial arch (ba2), changes in NCC behaviors and phenotypic consequences, and whether neuropilins work together to facilitate entry into and invasion of ba2. We find that cranial NCCs with reduced neuropilin-1 expression displayed shorter protrusions and decreased cell body and nuclear length-to-width ratios characteristic of a loss in polarity and motility, after specific interaction with ba2. Directed NCC migration was rescued by transplantation of transfected NCCs into rhombomere 4 of younger hosts. Lastly, reduction of neuropilin-2 expression by shRNA either solely or with reduction of neuropilin-1 expression did not lead to a stronger head phenotype. Thus, NCCs, independent of rhombomere origin, require neuropilin-1, but not neuropilin-2 to maintain polarity and directed migration into ba2.
Authors:
Rebecca McLennan; Paul M Kulesa
Related Documents :
2464073 - Distribution and function of tenascin during cranial neural crest development in the ch...
12788503 - Identification of human cells in brain xenografts and in neural co-cultures of rat by i...
23314043 - Effects of iodonium-class flavin dehydrogenase inhibitors on growth, reactive oxygen pr...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  239     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-26     Completed Date:  2010-09-30     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1664-73     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Branchial Region / metabolism*
Cell Movement
Chick Embryo
Embryo, Nonmammalian
Head
Neurons / metabolism*
Neuropilin-1 / metabolism*
Neuropilin-2
Neuropilins / metabolism
Phenotype
Signal Transduction
Skull / metabolism
Syndrome
Grant Support
ID/Acronym/Agency:
1R01HD057922/HD/NICHD NIH HHS; R01 HD057922/HD/NICHD NIH HHS; R01 HD057922-01A1/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Neuropilin-2; 0/Neuropilins; 144713-63-3/Neuropilin-1
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Draxin is involved in the proper development of the dI3 interneuron in chick spinal cord.
Next Document:  Dynamic expression pattern of Sonic hedgehog in developing cochlear spiral ganglion neurons.