Document Detail


Neuropeptides control the dynamic behavior of airway mucosal dendritic cells.
MedLine Citation:
PMID:  23049899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The airway mucosal epithelium is permanently exposed to airborne particles. A network of immune cells patrols at this interface to the environment. The interplay of immune cells is orchestrated by different mediators. In the current study we investigated the impact of neuronal signals on key functions of dendritic cells (DC). Using two-photon microscopic time-lapse analysis of living lung sections from CD11c-EYFP transgenic mice we studied the influence of neuropeptides on airway DC motility. Additionally, using a confocal microscopic approach, the phagocytotic capacity of CD11c(+) cells after neuropeptide stimulation was determined. Electrical field stimulation (EFS) leads to an unspecific release of neuropeptides from nerves. After EFS and treatment with the neuropeptides vasoactive intestinal peptide (VIP) or calcitonin gene-related peptide (CGRP), airway DC in living lung slices showed an altered motility. Furthermore, the EFS-mediated effect could partially be blocked by pre-treatment with the receptor antagonist CGRP(8-37). Additionally, the phagocytotic capacity of bone marrow-derived and whole lung CD11c(+) cells could be inhibited by neuropeptides CGRP, VIP, and Substance P. We then cross-linked these data with the in vivo situation by analyzing DC motility in two different OVA asthma models. Both in the acute and prolonged OVA asthma model altered neuropeptide amounts and DC motility in the airways could be measured. In summary, our data suggest that neuropeptides modulate key features motility and phagocytosis of mouse airway DC. Therefore altered neuropeptide levels in airways during allergic inflammation have impact on regulation of airway immune mechanisms and therefore might contribute to the pathophysiology of asthma.
Authors:
Sabrina Voedisch; Sabine Rochlitzer; Tibor Z Veres; Emma Spies; Armin Braun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-26
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-10     Completed Date:  2013-05-07     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e45951     Citation Subset:  IM    
Affiliation:
Department of Airway Immunology, Fraunhofer Institute for Toxicology and Experimental Medicine, Member of the German Center for Lung Research, Hannover, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD11c / biosynthesis
Asthma / metabolism
Bone Marrow Cells / cytology
Bronchi / metabolism
Dendritic Cells / cytology*
Hypersensitivity / metabolism
Immune System
Inflammation / metabolism
Lung / metabolism
Mice
Mice, Transgenic
Microscopy / methods
Microscopy, Confocal / methods
Mucous Membrane / cytology*
Neurons / metabolism
Neuropeptides / chemistry,  pharmacology*
Phagocytosis
Substance P / metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD11c; 0/Neuropeptides; 33507-63-0/Substance P
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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