Document Detail

Neuropeptide Y secretion from bovine chromaffin cells inhibits cyclic AMP accumulation.
MedLine Citation:
PMID:  12659167     Owner:  NLM     Status:  MEDLINE    
Neuropeptide Y (NPY) is secreted from bovine chromaffin cells in response to nicotinic receptor stimulation and may exhibit autocrine, paracrine or endocrine effects. Stimulation of bovine chromaffin cells with nicotine followed by the addition of forskolin (FSK) to the media results in a decrease in cyclic AMP accumulation compared to that seen in the absence of nicotine. Pertussis toxin (PTX) treatment or the addition of BIBP 3226, a selective NPY Y1 receptor antagonist prevents the inhibitory effect of nicotine. Fractionation of media obtained from cells stimulated with nicotine reveals an NPY-like substance that inhibits FSK-stimulated cAMP accumulation. Thus, an NPY-like substance can be secreted from bovine chromaffin cells in quantities sufficient to inhibit FSK-stimulated cAMP accumulation. These results suggest that NPY can act in an autocrine fashion to regulate chromaffin cell function.
J Zheng; G Zhou; T D Hexum
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Life sciences     Volume:  67     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2003-03-27     Completed Date:  2003-04-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  617-25     Citation Subset:  IM    
University of Nebraska Medical Center, Department of Pharmacology, 986260 Nebraska Medical Center, Omaha, NE 68198-6260, USA.
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MeSH Terms
Arginine / analogs & derivatives*,  pharmacology
Cells, Cultured
Chromaffin Cells / metabolism*
Chromatography, High Pressure Liquid
Culture Media, Conditioned
Cyclic AMP / metabolism*
Forskolin / antagonists & inhibitors
Neuropeptide Y / metabolism*
Nicotine / antagonists & inhibitors,  pharmacology
Nicotinic Agonists / pharmacology
Nicotinic Antagonists / pharmacology
Pertussis Toxin / pharmacology
Receptors, Neuropeptide Y / antagonists & inhibitors
Reg. No./Substance:
0/BIBP 3226; 0/Culture Media, Conditioned; 0/Neuropeptide Y; 0/Nicotinic Agonists; 0/Nicotinic Antagonists; 0/Receptors, Neuropeptide Y; 54-11-5/Nicotine; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 74-79-3/Arginine; EC Toxin

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