Document Detail


Neuropeptide Y receptor binding sites in rat brain: differential autoradiographic localizations with 125I-peptide YY and 125I-neuropeptide Y imply receptor heterogeneity.
MedLine Citation:
PMID:  2549216     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuropeptide Y (NPY) receptor binding sites have been localized in the rat brain by in vitro autoradiography using picomolar concentrations of both 125I-NPY and 125I-peptide YY (PYY) and new evidence provided for differentially localized receptor subtypes. Equilibrium binding studies using membranes indicate that rat brain contains a small population of high-affinity binding sites and a large population of moderate-affinity binding sites. 125I-PYY (10 pM) is selective for high-affinity binding sites (KD = 23 pM), whereas 10 pM 125I-NPY labels both high- and moderate-affinity sites (KD = 54 pM and 920 pM). The peptide specificity and affinity of these ligands in autoradiographic experiments match those seen in homogenates. Binding sites for 125I-PYY are most concentrated in the lateral septum, stratum oriens, and radiatum of the hippocampus, amygdala, piriform cortex, entorhinal cortex, several thalamic nuclei, including the reuniens and lateral posterior nuclei, and substantia nigra, pars compacta, and pars lateralis. In the brain stem, 125I-PYY sites are densest in a variety of nuclei on the floor of the fourth ventricle, including the pontine central grey, the supragenual nucleus, and the area postrema. 125I-NPY binding sites are found in similar areas, but relative levels of NPY binding and PYY binding differ regionally, suggesting differences in sites labeled by the two ligands. These receptor localizations resemble the distribution of endogenous NPY in some areas, but others, such as the hypothalamus, contain NPY immunoreactivity but few binding sites.
Authors:
D R Lynch; M W Walker; R J Miller; S H Snyder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  9     ISSN:  0270-6474     ISO Abbreviation:  J. Neurosci.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-09-22     Completed Date:  1989-09-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2607-19     Citation Subset:  IM    
Affiliation:
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Binding Sites
Brain / metabolism*
Iodine Radioisotopes / diagnostic use
Neuropeptide Y / metabolism
Peptide YY
Peptides / metabolism
Rats
Receptors, Neuropeptide Y
Receptors, Neurotransmitter / metabolism*
Tissue Distribution
Grant Support
ID/Acronym/Agency:
DA-00074/DA/NIDA NIH HHS; DA-00266/DA/NIDA NIH HHS; NS-16375/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Iodine Radioisotopes; 0/Neuropeptide Y; 0/Peptides; 0/Receptors, Neuropeptide Y; 0/Receptors, Neurotransmitter; 106388-42-5/Peptide YY

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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