Document Detail


Neuropeptide Y is an inducible gene in the human immune system.
MedLine Citation:
PMID:  8157736     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study reports on neuropeptide Y (NPY) mRNA expression in human peripheral blood mononuclear cells (PBMC) and lymphoid tissues. By reverse transcription polymerase chain reaction (RT-PCR) it is shown that activated human PBMC of normal blood donors expressed the NPY gene. The PCR products had the expected size and Northern blotting demonstrated the presence of the 0.8-kb NPY mRNA. To define the subpopulations of mononuclear cells expressing this neuropeptide, purified monocytes, B cells and T cells were stimulated with specific activators. Monocytes and in vitro matured macrophages expressed NPY mRNA in response to phorbol myristate acetate (PMA). B lymphocytes expressed NPY mRNA following stimulation with antibody to surface immunoglobulin and PMA. In order to analyze whether these cell types express NPY under physiological conditions in vivo, human bone marrow, tonsil and thymus were analyzed. In situ hybridization of bone marrow revealed a small number of cells containing high levels of NPY mRNA which was also detected in RNA extracts of human thymus and tonsil. In summary, NPY is an inducible gene in human lymphocytes and monocytes and it is expressed at sites where these cells are activated in vivo.
Authors:
H Schwarz; P M Villiger; J von Kempis; M Lotz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  51     ISSN:  0165-5728     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-05-13     Completed Date:  1994-05-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  53-61     Citation Subset:  IM    
Affiliation:
Sam and Rose Stein Institute for Research on Aging, University of California, San Diego, La Jolla 92093-0663.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow / metabolism
Bone Marrow Cells
Cell Line
Gene Expression Regulation*
Humans
Immune System / metabolism,  physiology*
In Situ Hybridization
Lymph Nodes / metabolism
Lymphocytes / metabolism
Monocytes / metabolism
Neuropeptide Y / blood,  genetics*
PC12 Cells / metabolism
Polymerase Chain Reaction
RNA, Messenger / blood
Rats
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
AR39799/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Neuropeptide Y; 0/RNA, Messenger

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