| Neuropeptide Y is expressed by rat mononuclear blood leukocytes and strongly down-regulated during inflammation. | |
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MedLine Citation:
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PMID: 18981110 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neuropeptide Y (NPY), a classical sympathetic comediator, regulates immunological functions including T cell activation and migration of blood leukocytes. A NPY-mediated neuroimmune cross-talk is well conceivable in sympathetically innervated tissues. In denervated, e.g., transplanted organs, however, leukocyte function is not fundamentally disturbed. Thus, we hypothesized that NPY is expressed by blood leukocytes themselves and regulated during inflammation. NPY mRNA and peptide expression were analyzed in mononuclear leukocytes isolated from the blood vessels of healthy rat kidneys, as well as from the blood vessels of isogeneic and allogeneic renal grafts transplanted in the Dark Agouti to Lewis or in the Fischer 344 to Lewis rat strain combination. Depending on the donor strain, acute allograft rejection is either fatal or reversible but both experimental models are characterized by massive accumulation of intravascular leukocytes. Leukocytes, predominantly monocytes, isolated from the blood vessels of untreated kidneys and isografts expressed high amounts of NPY mRNA and peptide, similar to expression levels in sympathetic ganglia. During acute allograft rejection, leukocytic NPY expression drastically dropped to approximately 1% of control levels in both rat strain combinations. In conclusion, NPY is an abundantly produced and tightly regulated cytokine of mononuclear blood leukocytes. |
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Authors:
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Julia Holler; Anna Zakrzewicz; Andreas Kaufmann; Jochen Wilhelm; Gabriele Fuchs-Moll; Hartmut Dietrich; Winfried Padberg; Jitka Kuncová; Wolfgang Kummer; Veronika Grau |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 181 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2008 Nov |
Date Detail:
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Created Date: 2008-11-04 Completed Date: 2008-12-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 6906-12 Citation Subset: AIM; IM |
Affiliation:
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Department of General and Thoracic Surgery, Laboratory of Experimental Surgery, University of Giessen Lung Center, Justus-Liebig-University Giessen, Giessen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Down-Regulation Flow Cytometry Gene Expression / immunology* Graft Rejection / immunology Immunohistochemistry Inflammation / immunology, metabolism* Kidney Transplantation Leukocytes, Mononuclear / immunology, metabolism* Male Neuropeptide Y / biosynthesis* RNA, Messenger / analysis Radioimmunoassay Rats Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Neuropeptide Y; 0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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