| Neurons are replenished in cultures of embryonic chick optic tectum after immunomagnetic depletion. | |
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MedLine Citation:
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PMID: 1864457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of intercellular interactions on the determination and differentiation of early embryonic brain cells was tested by immunomagnetic cell separation techniques. Using the A2B5 monoclonal antibody, which in chick brain reacts with a neuron-specific surface ganglioside, we produced initially pure populations of optic tectum cells devoid of the antigen. A coincident depletion of neurofilament(+) cells (95%) and nonneuronal growth characteristics of the separated A2B5(-) cells indicated that the vast majority of neurons had been removed initially. Surprisingly, A2B5(+) cells rapidly appeared in separated A2B5(-) cell cultures. After 1 day, the percentage of A2B5(+) cells in separated cell cultures equalled those in unseparated cultures (approximately 50%). By a week in culture, A2B5(+) cells developed neuronal morphology and contained neurofilaments. A2B5(-) to (+) conversion was a regulated phenomenon in that removal of different proportions of the (+) cells resulted in different numbers of (-) to (+) conversions. New DNA synthesis was not required for the acquisition of cell surface A2B5 antigen or for differentiation of cells into definitive A2B5(+) neurons. Our results demonstrate that postmitotic embryonic brain contains cells which are capable of replacing depleted neurons in vitro. |
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Authors:
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D S Galileo; A P Gee; P J Linser |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Developmental biology Volume: 146 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 1991 Aug |
Date Detail:
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Created Date: 1991-09-09 Completed Date: 1991-09-09 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 278-91 Citation Subset: IM |
Affiliation:
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Whitney Laboratory, University of Florida, St. Augustine 32086. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology Cell Differentiation Cell Separation* Cells, Cultured Chick Embryo / cytology*, physiology DNA / biosynthesis Gangliosides / immunology Immunologic Techniques Neuroglia / cytology, immunology Neurons / cytology, immunology, physiology* Oligodendroglia / immunology Recombination, Genetic Superior Colliculi / cytology, embryology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Gangliosides; 0/ganglioside A2B5; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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