| Neuronal potential of cells in early postnatal rat sciatic nerve. | |
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MedLine Citation:
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PMID: 8293877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A population of undifferentiated cells with neuronal potentialities were revealed in rat sciatic nerve. Explant cultures of sciatic nerve were prepared from newborn or early postnatal rat. Cultures were growth in F14 medium supplemented with 10% of fetal calf serum, incubated in a humidified 3% CO2, 97% air atmosphere. Within 2 weeks, refractile cells exhibiting the morphology of neurons were observed in all examined cultures. These cells had ovoid or multipolar refractile cells bodies with extended cytoplasmic processes. The neuronal nature of these cells was confirmed by their immunostaining with specific neuronal markers: neurofilament triplets, neuron-specific enolase, peripherin, microtubule-associated proteins, and brain spectrin. This neuronal population displayed various phenotypes. The CO2 concentration in the incubator plays an important role, since the number of differentiated neurons was lower in cultures incubated in 5% CO2. Since the sciatic nerve is devoid of nerve cell bodies in vivo, we concluded that early postnatal sciatic nerve contains crest cells with neuronal potentialities differentiating into neurons in response to the culture's environmental cues. |
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Authors:
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I B Walter |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Developmental biology Volume: 161 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-02-25 Completed Date: 1994-02-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 263-73 Citation Subset: IM |
Affiliation:
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Institut d'Histologie et d'Embryologie, Faculté de Médecine, Université de Lausanne, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbon Dioxide / pharmacology Cell Differentiation Cell Survival Cells, Cultured Neurons / physiology* Rats Rats, Wistar Sciatic Nerve / cytology* |
| Chemical | |
Reg. No./Substance:
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124-38-9/Carbon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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