Document Detail

Neuronal polarity: essential role of protein-lipid complexes in axonal sorting.
MedLine Citation:
PMID:  9520476     Owner:  NLM     Status:  MEDLINE    
The viral glycoprotein hemagglutinin (HA) and the endogenous glycosylphosphatidylinositol-anchored protein Thy-1 are efficiently targeted to the axonal surface of fully polarized hippocampal neurons in culture. Here we have shown that in these cells HA and Thy-1 interact with sphingolipid-cholesterol rafts and are included in detergent-insoluble glycolipid-enriched complexes. Axonal HA and Thy-1, but not two dendritic membrane proteins, resisted extraction to detergents at 4 degrees C. Both HA and Thy-1 became detergent-soluble in neurons with reduced levels of cholesterol or sphingolipids. Missorting of the axonal Thy-1 but not of a dendritic membrane protein occurred in sphingolipid-deprived cells. These results indicate that neurons sort a subset of axolemmal proteins by a mechanism that requires the formation of protein-lipid rafts. The involvement of rafts in axonal membrane sorting may explain the neurological deficits observed in patients with certain types of Niemann-Pick disease.
M D Ledesma; K Simons; C G Dotti
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  95     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-05-01     Completed Date:  1998-05-01     Revised Date:  2013-04-16    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3966-71     Citation Subset:  IM    
Cell Biology Program, European Molecular Biology Laboratory, 69112 Heidelberg, Germany.
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MeSH Terms
Antigens, Thy-1 / physiology*
Axons / physiology
Cell Membrane / physiology
Cell Polarity*
Cells, Cultured
Hemagglutinins / physiology*
Hippocampus / cytology*
Neurons / cytology*,  physiology
Sphingolipids / physiology*
Reg. No./Substance:
0/Antigens, Thy-1; 0/Hemagglutinins; 0/Sphingolipids

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