Document Detail


Neuronal nitric oxide synthase expression in developing and adult human CNS.
MedLine Citation:
PMID:  10068309     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuronal nitric oxide synthase (nNOS) is constitutively expressed by subpopulations of neurons in the CNS and is involved in neurotransmission, learning and memory, and neuronal injury. While the distribution of nNOS neurons has been characterized in the rodent CNS, the expression in human brain has not been well documented. We determined the expression of nNOS in second trimester human fetal and adult brain. In second trimester fetal brain, the nNOS neurons are concentrated in the developing cerebral cortex at the subplate zone and in layer VI, the striatum, and in certain brainstem nuclei. The nNOS neurons are sparsely distributed in the hippocampus, and virtually absent in the cerebellar cortex. The nNOS neurons in the subplate zone extend their processes radially, suggesting a developmental role, perhaps in guidance. The number and distribution of NADPH diaphorase-positive neurons corresponds to that of the nNOS neurons. While the distribution of nNOS neurons in the adult brain is similar to that found in fetal brain, the overall density is lower in the adult. The highest density of nNOS neurons is found in the striatum followed by the neocortex. A region-specific role for nNOS neurons in human brain and a potential developmental role for nNOS in the cerebral cortex are suggested by these data.
Authors:
M Downen; M L Zhao; P Lee; K M Weidenheim; D W Dickson; S C Lee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of neuropathology and experimental neurology     Volume:  58     ISSN:  0022-3069     ISO Abbreviation:  J. Neuropathol. Exp. Neurol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-26     Completed Date:  1999-02-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985192R     Medline TA:  J Neuropathol Exp Neurol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  12-21     Citation Subset:  IM    
Affiliation:
Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Brain / anatomy & histology,  physiology*
Embryonic and Fetal Development / physiology
Female
Histocytochemistry
Humans
Immunohistochemistry
Isoenzymes / biosynthesis*
Male
NADPH Dehydrogenase / metabolism
Neurons / enzymology*
Nitric Oxide Synthase / biosynthesis*
Grant Support
ID/Acronym/Agency:
MH55477/MH/NIMH NIH HHS; RG2771/RG/CSR NIH HHS; TG-NS07098/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.6.99.1/NADPH Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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